Güngör I H, Özer Kaya Ş, Dayan Cinkara S, Çakır Cihangiroğlu A, Kaya E, Acısu T C, Erdem Erişir F, Yılmaz Ö, Yılmaz S, Gür S, Sönmez M, Türk G
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey.
Iran J Vet Res. 2024;25(3):250-260. doi: 10.22099/ijvr.2024.49410.7253.
Cryopreservation of ram semen is a very challenging process. Loss of motility during freezing does not allow artificial insemination of rams.
This study aimed to determine whether the inclusion of liquid propolis extract in semen diluents affects the cryopreservation efficiency of ram semen.
Six Akkaraman breed rams were considered for semen study. Semens were combined with Tris+egg yolk extender containing and without (control) propolis at different concentrations (0.25%, 0.50%, 1%, 2%, and 4%). Semen was frozen using routine ram semen freezing procedures. After thawing, motility and kinematic parameters were analyzed by computer assisted semen analysis (CASA), and viability, acrosomal damage level and mitochondrial membrane potential were analyzed by flow-cytometer in all groups. Additionally, fatty acid levels in total semen were analyzed using gas chromatography (GC), and vitamin and cholesterol levels were analyzed using high-performance liquid chromatography (HPLC). In addition, oxidative stress, HOS test, and morphological analyzes were performed after freezing and thawing.
The 0.5% propolis group showed a significant increase in total and rapid motility, LIN, membrane integrity, and antioxidant levels compared to the control, and a significant decrease in malondialdehyde (MDA) and low mitochondrial membrane potential (LMMP) levels. Compared to the control, the group containing 4% propolis damaged spermatozoa and caused a significant decrease in total, progressive and rapid motility and high mitochondrial membrane potential (HMMP), Glutathione S-transferase (GST), and catalase (CAT) levels.
We showed that adding 0.5% propolis to semen extenders to increase the freezability level of ram semen increases the survival of spermatozoa after freeze-thaw and ensures the success of freezing.
冷冻保存公羊精液是一个极具挑战性的过程。冷冻过程中活力的丧失使得无法对公羊进行人工授精。
本研究旨在确定精液稀释剂中添加液体蜂胶提取物是否会影响公羊精液的冷冻保存效率。
选取6只阿卡拉曼品种公羊进行精液研究。精液分别与含有不同浓度（0.25%、0.50%、1%、2%和4%）蜂胶的Tris+蛋黄稀释剂以及不含蜂胶的（对照）稀释剂混合。采用常规的公羊精液冷冻程序对精液进行冷冻。解冻后，通过计算机辅助精液分析（CASA）分析活力和运动学参数，并通过流式细胞仪分析所有组的活力、顶体损伤水平和线粒体膜电位。此外，使用气相色谱（GC）分析总精液中的脂肪酸水平，使用高效液相色谱（HPLC）分析维生素和胆固醇水平。另外，在冷冻和解冻后进行氧化应激、HOS试验和形态学分析。
与对照组相比，0.5%蜂胶组的总活力和快速活力、直线性、膜完整性以及抗氧化水平显著提高，丙二醛（MDA）和低线粒体膜电位（LMMP）水平显著降低。与对照组相比，含有4%蜂胶的组对精子造成损伤，导致总活力、渐进性活力和快速活力以及高线粒体膜电位（HMMP）、谷胱甘肽S-转移酶（GST）和过氧化氢酶（CAT）水平显著降低。
我们表明，在精液稀释剂中添加0.5%蜂胶以提高公羊精液的冷冻能力，可增加冻融后精子的存活率，并确保冷冻成功。
