São Paulo State University (UNESP), School of Sciences, Humanities and Languages Assis, Department of Biotechnology, Laboratory of Herbal Medicine and Natural Products, Avenida Dom Antônio 2100, CEP: 19806-900, Assis, São Paulo, Brazil; São Paulo State University (UNESP), Institute of Chemistry, Rua Prof. Francisco Degni 55, CEP: 14800-060, Araraquara, São Paulo, Brazil.
Department of Botany, Ahmadu Bello University, Zaria, 810001 Nigeria.
Aquat Toxicol. 2019 Jun;211:81-91. doi: 10.1016/j.aquatox.2019.03.011. Epub 2019 Apr 1.
The excessive proliferation of toxin producing cyanobacteria constitutes a significant health risk to the environment and humans. This is due to the contamination of potable water and accumulation of cyanotoxins in plant and animal tissues. As a means of controlling bloom forming cyanobacteria, secondary metabolites with pro-oxidative activities from plants are used to treat water bodies contaminated with cyanobacterial blooms and their associated toxins. The objective of the present study was to evaluate the mechanism of action of extract, fractions and isolated flavonoids of Tridax procumbens L. on Microcystis aeruginosa (Kützing) Kützing. by monitoring changes in growth, oxidative stress, antioxidant response, and cyanatoxin microcystins (MCs) production. The extract, fraction 3 and the isolated flavonoids significantly reduced the cell density of the cyanobacterium. Furthermore, the extract and fraction 3 increased the production of reactive oxygen species, induced lipid peroxidation, and altered antioxidant enzyme activities of M. aeruginosa. The total MCs content of the cyanobacterium was negatively affected by the presence of the extract, fractions and isolated flavonoids. The present study show that T. procumbens has secondary metabolites that are capable of interfering with the physiology and microcystins production of M. aeruginosa. These characteristics are promising for the control of this noxious cyanobacterium in aquatic ecosystems.
产毒蓝藻的过度增殖对环境和人类构成了重大的健康风险。这是由于饮用水的污染和植物和动物组织中蓝藻毒素的积累。作为控制形成水华的蓝藻的一种手段，具有促氧化活性的植物次生代谢产物被用于处理受蓝藻水华及其相关毒素污染的水体。本研究的目的是通过监测生长、氧化应激、抗氧化反应和蓝藻毒素（MCs）产生的变化，评估三达龙（Tridax procumbens L.）提取物、馏分和分离的类黄酮对铜绿微囊藻（Kützing）Kützing 的作用机制。提取物、馏分 3 和分离的类黄酮显著降低了蓝藻的细胞密度。此外，提取物和馏分 3 增加了活性氧的产生，诱导了脂质过氧化，并改变了铜绿微囊藻的抗氧化酶活性。蓝藻总 MCs 含量受到提取物、馏分和分离类黄酮的存在的负面影响。本研究表明，三达龙含有能够干扰铜绿微囊藻生理和微囊藻毒素产生的次生代谢产物。这些特性有望控制水生生态系统中这种有害的蓝藻。
