Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore Campus, Lahore, Pakistan.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan.
Endocr Metab Immune Disord Drug Targets. 2022;22(14):1390-1402. doi: 10.2174/1871530322666220429125241.
The plant Berberis aristata is traditionally used and scientifically validated for treating obesity and hyperlipidemia. It is also traditionally used to treat gynecological abnormalities. Therefore, the present study was designed to evaluate the therapeutic potential of Berberis aristata for obesity-related reproductive changes and chemically characterize it.
High-fat diet was given to 36 female rats for six weeks to induce obesity and infertility. These obese rats were treated with 10 mg/kg orlistat or the plant extract at 125-500 mg/kg for 45 days.
The GC-MS analysis of the plant extract included fructose, thymic acid and other hydrocarbons. The plant extract revealed a remarkable free radical scavenging activity. The treated animals exhibited a decrease in total cholesterol and triglycerides (p<0.001), insulin and leptin levels (p<0.05), visceral fat, and body weight while increasing the estradiol level at 500 mg/kg dose of the plant extract as compared with untreated animals as demonstrated from the histology of the ovary. Oxidative stress biomarkers such as superoxide dismutase, nitric oxide, malondialdehyde and reduced glutathione were significantly (p<0.01-0.001) ameliorated in treated rats.
B. aristata exhibited substantial potential against obesity-inducedreproductive damage in female rats by reducing oxidative stress and resistance to leptin and insulin.
传统上使用小檗属植物小檗来治疗肥胖症和高血脂症，并通过科学验证。它也被传统上用于治疗妇科异常。因此，本研究旨在评估小檗属植物治疗肥胖相关生殖变化的治疗潜力，并对其进行化学表征。
给 36 只雌性大鼠喂食高脂肪饮食 6 周，以诱导肥胖和不孕。这些肥胖大鼠用 10mg/kg 奥利司他或 125-500mg/kg 的植物提取物治疗 45 天。
植物提取物的 GC-MS 分析包括果糖、胸腺酸和其他碳氢化合物。植物提取物显示出显著的自由基清除活性。与未治疗的动物相比，接受治疗的动物的总胆固醇和甘油三酯（p<0.001）、胰岛素和瘦素水平（p<0.05）、内脏脂肪和体重下降，而 500mg/kg 剂量的植物提取物则增加了雌二醇水平，这从卵巢组织学中可以看出。超氧化物歧化酶、一氧化氮、丙二醛和还原型谷胱甘肽等氧化应激生物标志物在治疗大鼠中显著（p<0.01-0.001）得到改善。
小檗属植物通过降低氧化应激和对瘦素和胰岛素的抵抗力，对肥胖引起的雌性大鼠生殖损伤表现出显著的潜在作用。
