Center of Innovative Nanostructure & Nanodevices (COINN), Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak, Malaysia; Department of Mechanical Engineering, Universiti Teknology PETRONAS, 32610 Seri Iskandar, Perak, Malaysia.
Center of Advanced Intelligent Materials, Universiti Malaysia Pahang, 26300 Kuantan, Pahang Darul Makmur, Malaysia; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Kuantan, Pahang Darul Makmur, Malaysia.
Food Chem. 2022 Oct 1;390:133105. doi: 10.1016/j.foodchem.2022.133105. Epub 2022 Apr 28.
Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (TiCT/PVDF) nanofiber composite is presented. Addition of TiCT up to 13% effectively increased the fiber diameter and lowered the β-phase of PVDF nanofibers, consequently lowering the charge transfer resistance. The nanofiber composite is then coated on the screen-printed carbon electrode to chemically functionalized with saline and aldehyde groups for efficient aptamer loading. The optimized aptasensor demonstrated sensitive detection of OTA over the dynamic concentration range from 1 fg mL to 1 ng mL with a limit of detection of 2.15 fg mL and quantification limit of 6.52 fg mL, with high selectivity. The aptasensor could detect the OTA at femtogram per milliliter concentration in grape juice samples, demonstrating its enormous potential for OTA detection in food industry.
赭曲霉毒素 A（OTA）对人类和动物健康有害；因此，对 OTA 的敏感和选择性检测有很高的要求。在此，提出了一种包含静电纺丝 MXene/聚偏二氟乙烯（TiCT/PVDF）纳米纤维复合材料的超灵敏电化学生物传感器电极。TiCT 的添加量增加到 13%时，纤维直径显著增大，同时降低了 PVDF 纳米纤维的β相，进而降低了电荷转移电阻。然后将纳米纤维复合材料涂覆在丝网印刷碳电极上，通过盐水和醛基进行化学功能化，以实现有效适配体加载。优化后的适体传感器在 1 fg mL 至 1 ng mL 的动态浓度范围内对 OTA 进行了灵敏检测，检测限为 2.15 fg mL，定量限为 6.52 fg mL，具有较高的选择性。该适体传感器可在葡萄汁样品中检测到飞克每毫升浓度的 OTA，展示了其在食品工业中检测 OTA 的巨大潜力。
