Lehrstuhl für Mikrobiologie, Technische Universität München (TUM), Gregor-Mendel-Straße 4, 85354 Freising, Germany.
Syst Appl Microbiol. 2022 Jul;45(4):126324. doi: 10.1016/j.syapm.2022.126324. Epub 2022 Apr 26.
Three moderately halophilic strains, TMW 2.2308, TMW 2.2299 and TMW 2.2304, were isolated from a lupine-based moromi fermentation. Initial identification based on their low molecular sub-proteome using mass spectrometry showed relation to the genus Halomonas, however, low score values indicated novelty. The comparison of 16S rRNA gene sequences placed these strains within the genus Chromohalobacter with C. japonicus CECT 7219 (99.67% 16S rRNA sequence similarity to strain TMW2.2308), C. canadensis DSM 6769 (99.54%) and C. beijerinckii LMG 2148 (99.32%) being their closest relatives. However, average nucleotide highest identity values of TMW 2.2308 to C. beijerinckii LMG 2148 of 93.12% and 92.88% to C. japonicus CECT 7219 demonstrate that it represents a novel species within the genus Chromohalobacter with additional strains TMW 2.2299 (96.91%) and TMW 2.2304 (96.98%). The isolated strains were non-spore-forming, motile and able to grow at temperatures from 5 to 45 °C with an optimum at 37 °C. Growth of TMW 2.2308 occurs at 5 to 25% (w/v) NaCl with optimum growth between 10and 12.5%. The genome of TMW 2.2308 has a size of 3.47 Mb and a G + C content of 61.0 mol%. The polyphasic evidence lead to the classification of TMW 2.2308, TMW 2.2299 and TMW 2.2304 as members of a novel species of the genus Chromohalobacter. We propose a novel species as Chromohalobacter moromii sp. nov., with TMW 2.2308 (=DSM113153 =CECT30422) as the type strain.
从基于羽扇豆的味噌发酵中分离出三株中度嗜盐菌株，TMW 2.2308、TMW 2.2299 和 TMW 2.2304。最初基于它们的低分子量亚蛋白组使用质谱进行的鉴定表明与 Halomonas 属有关，但低评分值表明这是新颖的。16S rRNA 基因序列的比较将这些菌株置于 Chromohalobacter 属内，与 C. japonicus CECT 7219（与菌株 TMW2.2308 的 16S rRNA 序列相似度为 99.67%）、C. canadensis DSM 6769（99.54%）和 C. beijerinckii LMG 2148（99.32%）最为接近。然而，TMW 2.2308 与 C. beijerinckii LMG 2148 的平均核苷酸最高同一性值为 93.12%，与 C. japonicus CECT 7219 的平均核苷酸最高同一性值为 92.88%，表明它代表 Chromohalobacter 属中的一个新种，此外还有菌株 TMW 2.2299（96.91%）和 TMW 2.2304（96.98%）。分离株是非孢子形成的、运动的，能够在 5 至 45°C 的温度下生长，最佳温度为 37°C。TMW 2.2308 的生长发生在 5 至 25%（w/v）NaCl 中，最佳生长在 10 至 12.5%之间。TMW 2.2308 的基因组大小为 3.47 Mb，G+C 含量为 61.0 mol%。多相证据导致 TMW 2.2308、TMW 2.2299 和 TMW 2.2304 被分类为 Chromohalobacter 属的一个新种。我们提出了一个新种，即 Chromohalobacter moromii sp. nov.，以 TMW 2.2308（=DSM113153=CECT30422）作为模式菌株。
