Department of Gene Structure and Function, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 24A, 50-363, Wrocław, Poland.
Sci Rep. 2022 May 17;12(1):8164. doi: 10.1038/s41598-022-12257-7.
Narrow-leafed lupin (NLL, Lupinus angustifolius L.) is a legume plant cultivated for grain production and soil improvement. Worldwide expansion of NLL as a crop attracted various pathogenic fungi, including Colletotrichum lupini causing a devastating disease, anthracnose. Two alleles conferring improved resistance, Lanr1 and AnMan, were exploited in NLL breeding, however, underlying molecular mechanisms remained unknown. In this study, European NLL germplasm was screened with Lanr1 and AnMan markers. Inoculation tests in controlled environment confirmed effectiveness of both resistance donors. Representative resistant and susceptible lines were subjected to differential gene expression profiling. Resistance to anthracnose was associated with overrepresentation of "GO:0006952 defense response", "GO:0055114 oxidation-reduction process" and "GO:0015979 photosynthesis" gene ontology terms. Moreover, the Lanr1 (83A:476) line revealed massive transcriptomic reprogramming quickly after inoculation, whereas other lines showed such a response delayed by about 42 h. Defense response was associated with upregulation of TIR-NBS, CC-NBS-LRR and NBS-LRR genes, pathogenesis-related 10 proteins, lipid transfer proteins, glucan endo-1,3-beta-glucosidases, glycine-rich cell wall proteins and genes from reactive oxygen species pathway. Early response of 83A:476, including orchestrated downregulation of photosynthesis-related genes, coincided with the successful defense during fungus biotrophic growth phase, indicating effector-triggered immunity. Mandelup response was delayed and resembled general horizontal resistance.
窄叶羽扇豆（NLL，Lupinus angustifolius L.）是一种豆科植物，用于谷物生产和土壤改良。NLL 在全球范围内的种植扩展吸引了各种致病真菌，包括引起毁灭性疾病炭疽病的炭疽菌（Colletotrichum lupini）。利用Lanr1 和 AnMan 这两个等位基因赋予了 NLL 改良的抗性，然而，其潜在的分子机制仍不清楚。本研究利用 Lanr1 和 AnMan 标记物对欧洲 NLL 种质资源进行了筛选。在受控环境下的接种试验证实了两个抗性供体的有效性。选择有代表性的抗性和敏感系进行差异基因表达谱分析。对炭疽病的抗性与“GO：0006952 防御反应”、“GO：0055114 氧化还原过程”和“GO：0015979 光合作用”等基因本体论术语的过度表达有关。此外，Lanr1（83A:476）系在接种后迅速发生大规模转录组重编程，而其他系则在大约 42 小时后才出现这种反应。防御反应与 TIR-NBS、CC-NBS-LRR 和 NBS-LRR 基因、病程相关蛋白 10、脂质转移蛋白、葡聚糖内切-1,3-β-葡萄糖苷酶、富含甘氨酸的细胞壁蛋白和活性氧途径基因的上调有关。83A:476 的早期反应，包括光合作用相关基因的协调下调，与真菌生物营养生长阶段成功的防御相吻合，表明了效应物触发的免疫。Mandelup 反应被延迟，类似于一般的水平抗性。
