Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.
Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.
Plant Cell Environ. 2020 Nov;43(11):2680-2698. doi: 10.1111/pce.13880. Epub 2020 Sep 23.
The narrow-leafed lupin, Lupinus angustifolius L., is a grain legume crop, cultivated both as a green manure and as a source of protein for animal feed and human food production. During its domestication process, numerous agronomic traits were improved, however, only two trait-related genes were identified hitherto, both by linkage mapping. Genome-wide association studies (GWAS), exploiting genomic sequencing, did not select any novel candidate gene. In the present study, an innovative method of 3'-end reduced representation transcriptomic profiling, a massive analysis of cDNA ends, has been used for genotyping of 126 L. angustifolius lines surveyed by field phenotyping. Significant genotype × environment interactions were identified for all phenology and yield traits analysed. Principal component analysis of population structure evidenced European domestication bottlenecks, visualized by clustering of breeding materials and cultivars. GWAS provided contribution towards deciphering vernalization pathway in legumes, and, apart from highlighting known domestication loci (Ku/Julius and mol), designated novel candidate genes for L. angustifolius traits. Early phenology was associated with genes from vernalization, cold-responsiveness and phosphatidylinositol signalling pathways whereas high yield with genes controlling photosynthesis performance and abiotic stress (drought or heat) tolerance. PCR-based toolbox was developed and validated to enable tracking desired alleles in marker-assisted selection. Narrow-leafed lupin was genotyped with an innovative method of transcriptome profiling and phenotyped for phenology, growth and yield traits in field. Early phenology was found associated with genes from cold-response, vernalization and phosphatidylinositol signalling pathways, whereas high yield with genes running photosystem II and drought or heat stress response. Key loci were supplied with PCR-based toolbox for marker-assisted selection.
窄叶羽扇豆（ Lupinus angustifolius L. ）是一种粮用豆科作物，既作为绿肥种植，也作为动物饲料和人类食品生产的蛋白质来源。在其驯化过程中，许多农艺性状得到了改良，但迄今为止仅通过连锁作图鉴定出了两个与性状相关的基因。利用基因组测序进行的全基因组关联研究（GWAS）也没有选择任何新的候选基因。在本研究中，采用了一种创新的 3'末端减少代表性转录组谱分析方法，即大规模 cDNA 末端分析，对通过田间表型调查的 126 个窄叶羽扇豆品系进行了基因分型。所有分析的物候和产量性状均鉴定出显著的基因型与环境互作。基于群体结构的主成分分析证明了欧洲驯化瓶颈的存在，这是通过对育种材料和品种的聚类可视化的。GWAS 有助于解析豆科植物的春化途径，除了突出已知的驯化基因座（Ku/Julius 和 mol）外，还指定了窄叶羽扇豆性状的新候选基因。早期物候与春化、冷响应和磷脂酰肌醇信号通路的基因有关，而高产量与控制光合作用性能和非生物胁迫（干旱或热）耐受性的基因有关。开发并验证了基于 PCR 的工具盒，以实现标记辅助选择中所需等位基因的跟踪。利用创新的转录组谱分析方法对窄叶羽扇豆进行了基因分型，并在田间对其物候、生长和产量性状进行了表型分析。早期物候与冷响应、春化和磷脂酰肌醇信号通路的基因有关，而高产量与运行光系统 II 以及干旱或热胁迫反应的基因有关。为了标记辅助选择，提供了带有基于 PCR 的工具盒的关键基因座。
