Interfacial Electrochemistry and Biomaterials, Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, Shanghai Engineering Research Center of Hierarchical Nanomaterials, School of Materials Science and Engineering, East China University of Science & Technology, Shanghai 200237, China.
Chair of Materials Science, Otto Schott Institute of Materials Research, Friedrich Schiller University Jena, 07743 Jena, Germany.
Mater Horiz. 2022 Jul 4;9(7):1962-1968. doi: 10.1039/d1mh02009a.
Directly targeting bacterial cells is the present paradigm for designing antimicrobial biomaterial surfaces and minimizing device-associated infections (DAIs); however, such pathways may create problems in tissue integration because materials that are toxic to bacteria can also be harmful to mammalian cells. Herein, we report an unexpected antimicrobial effect of calcium-doped titanium, which itself has no apparent killing effect on the growth of pathogenic bacteria (, , ATCC 27853) while presenting strong inhibition efficiency on bacterial colonization after fibrinogen adsorption onto the material. Fine X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy analyses reported calcium-dependent shifts of the binding energy in nitrogen and oxygen involved groups and wavenumbers in the amide I and II bands of the adsorbent fibrinogen, demonstrating that locally delivered calcium can react with the carboxy-terminal regions of the Aα chains and influence their interaction with the N-termini of the Bβ chains in fibrinogen. These reactions facilitate the exposure of the antimicrobial motifs of the protein, indicating the reason for the surprising antimicrobial efficacy of calcium-doped titanium. Since protein adsorption is an immediate intrinsic step during the implantation surgery, this finding may shift the present paradigm on the design of implantable antibacterial biomaterial surfaces.
直接针对细菌细胞是设计抗菌生物材料表面和最大限度减少器械相关感染 (DAIs) 的当前范例；然而，这种途径可能会在组织整合中产生问题，因为对细菌有毒的材料也可能对哺乳动物细胞有害。在此，我们报告了钙掺杂钛的意外抗菌作用，其本身对致病性细菌（金黄色葡萄球菌、表皮葡萄球菌、ATCC 27853）的生长没有明显的杀伤作用，而在纤维蛋白原吸附到材料上后，对细菌定植具有很强的抑制效率。精细 X 射线光电子能谱和傅里叶变换红外光谱分析报告了与氮和氧相关基团结合能以及吸附剂纤维蛋白原酰胺 I 和 II 带中波数的钙依赖性位移，表明局部递送的钙可以与 Aα 链的羧基末端区域反应，并影响它们与纤维蛋白原中 Bβ 链的 N 末端的相互作用。这些反应促进了蛋白质抗菌基序的暴露，表明了钙掺杂钛具有惊人抗菌功效的原因。由于蛋白质吸附是植入手术中立即发生的内在步骤，这一发现可能会改变目前对可植入抗菌生物材料表面设计的范例。
