姜黄素和百里醌联合抑制乳腺癌细胞系的进展。

Curcumin and Thymoquinone Combination Attenuates Breast Cancer Cell Lines' Progression.

机构信息

Damanhour University, Egypt.

University of Jeddah, Saudi Arabia.

出版信息

Integr Cancer Ther. 2022 Jan-Dec;21:15347354221099537. doi: 10.1177/15347354221099537.

Breast cancer is the most harmful malignancy in women worldwide. Therefore, in the current study, we investigated the combinatory effect of natural bioactive compounds, including curcumin (Cur) and thymoquinone (TQ), on MCF7 and MDA-MB-231 breast cancer cell lines' progression. We investigated the Fa values and combination index of Cur and TQ in this context. Moreover, cytotoxicity percentages, annexin-V, proliferation, colony formation, and migration assays were used along with cell cycle analysis. In addition, caspase-3, phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT) protein levels were determined by ELISA assessment. The results showed that Cur, TQ, and Cur + TQ induced apoptosis with cell cycle arrest and decreased cell proliferation, colony formation, and migration activities. Cur + TQ combination significantly increased caspase-3 and decreased PI3K and AKT protein levels. These results suggest the promising anticancer benefit of the Cur and TQ combination against breast cancer.

乳腺癌是全球女性最具危害性的恶性肿瘤。因此，在本研究中，我们研究了包括姜黄素（Cur）和百里醌（TQ）在内的天然生物活性化合物的组合效应对 MCF7 和 MDA-MB-231 乳腺癌细胞系进展的影响。我们在这方面研究了 Cur 和 TQ 的 Fa 值和组合指数。此外，还进行了细胞毒性百分比、Annexin-V、增殖、集落形成和迁移测定以及细胞周期分析。此外，通过 ELISA 评估测定了半胱天冬酶-3、磷酸肌醇 3-激酶（PI3K）和蛋白激酶 B（AKT）蛋白水平。结果表明，Cur、TQ 和 Cur+TQ 诱导细胞凋亡，导致细胞周期停滞，并降低细胞增殖、集落形成和迁移活性。Cur+TQ 联合显著增加了半胱天冬酶-3 并降低了 PI3K 和 AKT 蛋白水平。这些结果表明，Cur 和 TQ 的组合具有有希望的抗癌益处，可用于治疗乳腺癌。