Radenković Milena, Alkildani Said, Stoewe Ignacio, Bielenstein James, Sundag Bernd, Bellmann Olaf, Jung Ole, Najman Stevo, Stojanović Sanja, Barbeck Mike
Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.
BerlinAnalytix GmbH, 12109 Berlin, Germany.
Membranes (Basel). 2021 Sep 15;11(9):712. doi: 10.3390/membranes11090712.
Collagen-based resorbable barrier membranes have been increasingly utilized for Guided Bone Regeneration (GBR), as an alternative to non-resorbable synthetic membranes that require a second surgical intervention for removal. One of the most important characteristics of a resorbable barrier membrane is its mechanical integrity that is required for space maintenance and its tissue integration that plays a crucial role in wound healing and bone augmentation. This study compares a commercially available porcine-derived sugar-crosslinked collagen membrane with two non-crosslinked collagen barrier membranes. The material analysis provides an insight into the influence of manufacturing on the microstructure. In vivo subcutaneous implantation model provides further information on the host tissue reaction of the barrier membranes, as well as their tissue integration patterns that involve cellular infiltration, vascularization, and degradation. The obtained histochemical and immunohistochemical results over three time points (10, 30, and 60 days) showed that the tissue response to the sugar crosslinked collagen membrane involves inflammatory macrophages in a comparable manner to the macrophages observed in the surrounding tissue of the control collagen-based membranes, which were proven as biocompatible. The tissue reactions to the barrier membranes were additionally compared to wounds from a sham operation. Results suggest wound healing properties of all the investigated barrier membranes. However, the sugar-crosslinked membrane lacked in cellular infiltration and transmembraneous vascularization, providing an exclusive barrier function in GBR. Moreover, this membrane maintained a similar swelling ratio over examined timepoints, which suggests a very slow degradation pattern and supports its barrier function. Based on the study results, which showed biocompatibility of the sugar crosslinked membrane and its stability up to 60 days post-implantation, it can be concluded that this membrane may be suitable for application in GBR as a biomaterial with exclusive barrier functionality, similar to non-resorbable options.
基于胶原蛋白的可吸收屏障膜已越来越多地用于引导骨再生（GBR），作为需要二次手术取出的不可吸收合成膜的替代品。可吸收屏障膜最重要的特性之一是其维持空间所需的机械完整性及其在伤口愈合和骨增量中起关键作用的组织整合性。本研究将一种市售的猪源糖交联胶原蛋白膜与两种非交联胶原蛋白屏障膜进行了比较。材料分析深入了解了制造对微观结构的影响。体内皮下植入模型提供了关于屏障膜宿主组织反应的进一步信息，以及它们涉及细胞浸润、血管化和降解的组织整合模式。在三个时间点（10天、30天和60天）获得的组织化学和免疫组织化学结果表明，对糖交联胶原蛋白膜的组织反应涉及炎性巨噬细胞，其方式与在对照胶原蛋白基膜周围组织中观察到的巨噬细胞相当，后者已被证明具有生物相容性。还将屏障膜的组织反应与假手术伤口进行了比较。结果表明所有研究的屏障膜都具有伤口愈合特性。然而，糖交联膜缺乏细胞浸润和跨膜血管化，在GBR中提供了独特的屏障功能。此外，该膜在检查的时间点保持相似的肿胀率，这表明其降解模式非常缓慢并支持其屏障功能。基于研究结果，表明糖交联膜具有生物相容性且在植入后60天内保持稳定，可以得出结论，该膜可能适合作为具有独特屏障功能的生物材料应用于GBR，类似于不可吸收的材料。
