Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517 Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517 Japan; Department of Chemical Science and Engineering, National Institute of Technology, Miyakonojo College, 473-1 Yoshio-cho, Miyakonojo, Miyazaki 885-8567, Japan.
Water Res. 2022 Jul 1;219:118581. doi: 10.1016/j.watres.2022.118581. Epub 2022 May 10.
Polyethylene terephthalate (PET) is produced worldwide, mainly as material for plastic drink bottles. PET is produced by polymerization of purified terephthalate (PTA) or dimethyl terephthalate (DMT) with ethylene glycol. During the synthetic manufacturing processes of PTA and DMT, high organic loading wastewater is produced, which is typically treated separately by anaerobic wastewater treatment technologies. Given the high demand for PET, manufacturing plants are expanding globally, which will result in an increase in the amounts of PTA and DMT wastewater in need of treatment. In terms of effective treatment, the cotreatment of PTA and DMT wastewater has several advantages, including lower area and energy requirements. In this study, we examined the performance of an upflow anaerobic sludge blanket (UASB) reactor in cotreating PTA and DMT wastewater with high organic loading, evaluating its removal characteristics after 518 days of continuous operation. In addition, we performed a microbiome analysis of the UASB granular sludge to uncover the microbial interactions and metabolic functions within the reactor. By continuous operation, we achieved an organic removal rate of 6.6 kg m day. In addition, we confirmed that aromatic compounds in the complex wastewater from the PTA and DMT manufacturing processes are biodegradable in the following order: benzoate > orthophthalate > terephthalate > isophthalate > p-toluic acid. 16S rRNA gene-based network analysis shows that anaerobic Woesearchaeales belonging to phylum Nanoarchaeota has a positive correlation with Methanoregula, Candidatus Methanofastidiosum, and Methanosarcina, suggesting a symbiotic relationship with methanogens in granular sludge. Shotgun metagenomic analysis revealed that terephthalate, isophthalate/orthophthalate, and benzoate were degraded by different members of Pelotomaculaceae and Syntrophorhabdaceae. According to the genomic information, we propose two new possible routes for orthophthalate degradation by the Syntrophorhabdaceae organism.
聚对苯二甲酸乙二醇酯（PET）在全球范围内生产，主要用作塑料饮料瓶的材料。PET 通过对纯化对苯二甲酸（PTA）或对苯二甲酸二甲酯（DMT）与乙二醇的聚合反应来生产。在 PTA 和 DMT 的合成制造过程中，会产生高有机负荷废水，通常通过厌氧废水处理技术分别进行处理。鉴于对 PET 的高需求，制造工厂正在全球范围内扩张，这将导致需要处理的 PTA 和 DMT 废水的数量增加。在有效处理方面，PTA 和 DMT 废水的共处理具有几个优点，包括较低的占地面积和能源需求。在这项研究中，我们考察了上流式厌氧污泥床（UASB）反应器在高有机负荷下共处理 PTA 和 DMT 废水的性能，评估了在连续运行 518 天后的去除特性。此外，我们对 UASB 颗粒污泥进行了微生物组分析，以揭示反应器内的微生物相互作用和代谢功能。通过连续运行，我们实现了 6.6kgm-3d-1 的有机去除率。此外，我们证实了来自 PTA 和 DMT 制造过程的复杂废水中的芳香族化合物在以下顺序中是可生物降解的：苯甲酸盐>邻苯二甲酸盐>对苯二甲酸盐>间苯二甲酸盐>对甲苯酸。基于 16S rRNA 基因的网络分析表明，属于纳米古菌门的厌氧 Woesearchaeales 与 Methanoregula、Candidatus Methanofastidiosum 和 Methanosarcina 呈正相关，这表明在颗粒污泥中与产甲烷菌存在共生关系。鸟枪法宏基因组分析表明，对苯二甲酸、间苯二甲酸盐/邻苯二甲酸盐和苯甲酸盐分别由 Pelotomaculaceae 和 Syntrophorhabdaceae 的不同成员降解。根据基因组信息，我们提出了 Syntrophorhabdaceae 生物降解邻苯二甲酸盐的两种新的可能途径。
