Doytchinov Vesselin V, Peykov Slavil, Dimov Svetoslav G
Department of Genetics, Faculty of Biology, Sofia University "St. Kliment Ohridski", 1164 Sofia, Bulgaria.
Life (Basel). 2024 Feb 19;14(2):278. doi: 10.3390/life14020278.
As belonging to one of the most isolated continents on our planet, the microbial composition of different environments in Antarctica could hold a plethora of undiscovered species with the potential for biotechnological applications. This manuscript delineates our discoveries after an expedition to the Bulgarian Antarctic Base "St. Kliment Ohridski" situated on Livingston Island, Antarctica. Amplicon-based metagenomics targeting the 16S rRNA genes and ITS2 region were employed to assess the metagenomes of the bacterial, fungal, and archaeal communities across diverse sites within and proximal to the research station. The predominant bacterial assemblages identified included , , , and . A substantial proportion of cyanobacteria reads were attributed to a singular uncultured taxon within the family . The bacterial profile of a lagoon near the base exhibited indications of penguin activity, characterized by a higher abundance of , similar to lithotelm samples from Hannah Pt. Although most fungal reads in the samples could not be identified at the species level, noteworthy genera, namely and , were identified. Archaeal abundance was negligible, with prevalent groups including , , , and Marine Group II.
作为地球上最与世隔绝的大陆之一，南极洲不同环境中的微生物组成可能包含大量未被发现的物种，具有生物技术应用潜力。本手稿描述了我们在前往位于南极洲利文斯顿岛的保加利亚南极基地“圣克莱门特·奥赫里德斯基”考察后的发现。采用基于扩增子的宏基因组学方法，靶向16S rRNA基因和ITS2区域，以评估研究站内及附近不同地点细菌、真菌和古菌群落的宏基因组。鉴定出的主要细菌组合包括 、 、 和 。大量蓝细菌读数归因于 科内一个单一的未培养分类单元。基地附近一个泻湖的细菌谱显示出企鹅活动的迹象，其特征是 丰度较高，类似于汉娜角的岩石渗坑样本。尽管样本中的大多数真菌读数无法在物种水平上鉴定，但鉴定出了值得注意的属，即 和 。古菌丰度可忽略不计，主要类群包括 、 、 和海洋第二组。
