Institute of Innovative Biotechnology in Animal Husbandry - Branch of L.K. Ernst Federal Science Center for Animal Husbandry, Kostyakova str. 12 bld. 4, Moscow 127422, Russia.
Institute of Innovative Biotechnology in Animal Husbandry - Branch of L.K. Ernst Federal Science Center for Animal Husbandry, Kostyakova str. 12 bld. 4, Moscow 127422, Russia.
Virus Res. 2022 Aug;317:198813. doi: 10.1016/j.virusres.2022.198813. Epub 2022 May 15.
Enzootic bovine leukosis is one of the unsolved problems of cattle breeding in many countries. The etiological agent of the disease is the bovine leukemia virus (BLV) - an oncogenic retrovirus, that infects B-lymphocytes in cattle. The number and genetic content of BLV provirus integration sites in the bovine genome were reported to can be used as an early diagnostic sign of leukemogenesis in the infected cattle, but patterns of BLV provirus integration into the bovine genome and associations between genomic features of the integration sites and development of lymphocytosis and B-cell lymphomas remain poorly elucidated. Here we present data on five novel BLV provirus integration sites in the genome of cattle with persistent lymphocytosis. Two of these sites were located in introns of scfd2 and pgpep1 genes, which have been recognized as cancer driver genes. Three of the rest integration sites were found in the intergenic spaces between ctps1 and cited4, nampt and ccdc71, skp2 and lmbrd2 genes, from which cited4 and skp2 also possess oncogenic properties. These data support previous findings of the association between localization of BLV proviral DNA near cancer driver genes and leukemogenesis in the BLV-infected cattle.
牛传染性白血病是许多国家牛养殖中尚未解决的问题之一。该病的病原体是牛白血病病毒（BLV），一种致瘤性逆转录病毒，感染牛的 B 淋巴细胞。BLV 前病毒整合在牛基因组中的数量和遗传内容可作为感染牛白血病发生的早期诊断标志，但 BLV 前病毒整合到牛基因组中的模式以及整合部位的基因组特征与淋巴细胞增多和 B 细胞淋巴瘤的发展之间的关联仍未得到充分阐明。在这里，我们提供了关于持续性淋巴细胞增多牛的基因组中五个新的 BLV 前病毒整合部位的数据。其中两个位点位于 scfd2 和 pgpep1 基因的内含子中，这些基因已被认为是致癌驱动基因。其余三个整合部位位于 ctps1 和 cited4、nampt 和 ccdc71、skp2 和 lmbrd2 基因之间的基因间空间，其中 cited4 和 skp2 也具有致癌特性。这些数据支持 BLV 前病毒 DNA 定位于致癌驱动基因附近与 BLV 感染牛白血病发生之间存在关联的先前发现。
