Stevens L E, Durrwachter J R, Helton D O
J Pharm Sci. 1986 Jan;75(1):83-6. doi: 10.1002/jps.2600750120.
Two methods are described for the analysis of chlorhexidine sorption in soft contact lenses. The first is an isocratic ion-pairing high-performance liquid chromatographic (HPLC) method with UV detection at 220 nm that allows the determination of chlorhexidine, p-chloroaniline, and other chlorhexidine degradation products in ophthalmic solutions. This procedure had a detection limit of 0.1 ng. The second involves the catalytic oxidation of the lens matrix containing [14C]chlorhexidine to [14C]carbon dioxide and water. The label is then trapped as carbon dioxide in a cocktail and is analyzed by liquid scintillation counting. These methods are sensitive, accurate, and reproducible, and can be used independently or in conjunction for the determination of chlorhexidine sorption in soft contact lenses.
本文描述了两种用于分析软性隐形眼镜中洗必泰吸附情况的方法。第一种是等度离子对高效液相色谱（HPLC）法，在220 nm处进行紫外检测，可测定眼科溶液中的洗必泰、对氯苯胺和其他洗必泰降解产物。该方法的检测限为0.1 ng。第二种方法是将含有[14C]洗必泰的镜片基质催化氧化为[14C]二氧化碳和水。然后，标记物作为二氧化碳捕获在混合液中，并通过液体闪烁计数法进行分析。这些方法灵敏、准确且可重复，可单独使用或联合使用以测定软性隐形眼镜中洗必泰的吸附情况。
