Anywar Godwin Upoki, Kakudidi Esezah, Oryem-Origa Hannington, Schubert Andreas, Jassoy Christian
Department of Plant Sciences, Microbiology & Biotechnology, College of Natural Sciences, Makerere University, Kampala, Uganda.
Fraunhofer Institute for Cell Therapy & Immunology (IZI), Leipzig, Germany.
Front Toxicol. 2022 May 2;4:832780. doi: 10.3389/ftox.2022.832780. eCollection 2022.
Many people living with HIV/AIDS (PLHIV) in Uganda widely use herbal medicines. However, their toxicity and safety have not been investigated. The use of these plants can potentially cause harmful effects to the health of patients. The purpose of this study was to determine the cytotoxicity of some commonly used medicinal plant species used by PLHIV. The cytotoxicity of the plant extracts was determined with the AlamarBlue cell viability assay using the human glioblastoma cell line U87.CD4.CXCR4. The cells were treated with varying concentrations of extracts of , , , , , , , , , , and reconstituted with ethanol and dimethyl sulfoxide (DMSO). Using regression analysis, the half maximal cytotoxic concentration (CC) of the plant extracts were calculated from exponential curve fits, since they provided the highest coefficient of determination, R. The ethanol extracts of (CC = 7.6 μg/ml) and (CC = 1.5 μg/ml) as well as the DMSO-reconstituted extracts of (CC = 6.4 μg/ml) and (CC = < 4 μg/ml) were highly cytotoxic. The cytotoxicity of and compared well with the indigenous traditional knowledge of the toxic effects experienced when the plants were not used correctly. However, the cytotoxicity of most of the plant extracts (15/22) was low to moderate (CC = 21-200 μg/ml). Most of the plant species tested in this study had low to moderate cytotoxicity against U87.CD4.CXCR4 cells, except and which were highly cytotoxic.
乌干达许多感染艾滋病毒/艾滋病的人广泛使用草药。然而，其毒性和安全性尚未得到研究。使用这些植物可能会对患者健康造成有害影响。本研究的目的是确定感染艾滋病毒/艾滋病的人常用的一些药用植物物种的细胞毒性。使用人胶质母细胞瘤细胞系U87.CD4.CXCR4，通过AlamarBlue细胞活力测定法测定植物提取物的细胞毒性。用不同浓度的、、、、、、、、、、和的提取物处理细胞，这些提取物用乙醇和二甲基亚砜（DMSO）复溶。使用回归分析，从指数曲线拟合计算植物提取物的半数最大细胞毒性浓度（CC），因为它们提供了最高的决定系数R。（CC = 7.6μg/ml）和（CC = 1.5μg/ml）的乙醇提取物以及（CC = 6.4μg/ml）和（CC = < 4μg/ml）的DMSO复溶提取物具有高度细胞毒性。和的细胞毒性与不正确使用这些植物时所经历的毒性的本土传统知识相符。然而，大多数植物提取物（15/22）的细胞毒性为低至中度（CC = 21 - 200μg/ml）。本研究中测试的大多数植物物种对U87.CD4.CXCR4细胞的细胞毒性为低至中度，除了和具有高度细胞毒性。
