Ikehara K, Kakitani H, Ishino S, Okada Y
J Biochem. 1979 Mar;85(3):633-9.
Some properties of unprimed poly(A)-poly(U) synthesis by DNA-dependent RNA polymerase from Caulobacter crescentus were examined. The reaction required ATP and UTP as substrates and manganese as a divalent cation. Rifampicin completely inhibited the reaction at a concentration of 1 micron/ml, and the enzyme catalyzed the polymer synthesis well regardless of the presence of GTP, CTP or both. The chain length of the poly(A)-poly(U) synthesized was about one hundred base pairs, as estimated from a sedimentation velocity and the molar ratio of [3H]AMP to [gamma-32P]ATP incorporated into the poly(A)-poly(U). The reaction was dependent on the square of the enzyme concentration and the enzyme dimers formed complexes with poly(A)-poly(U) during the reaction.
研究了新月柄杆菌依赖DNA的RNA聚合酶进行的未引发的聚（A）-聚（U）合成的一些特性。该反应需要ATP和UTP作为底物以及锰作为二价阳离子。利福平在浓度为1微克/毫升时完全抑制该反应，并且无论是否存在GTP、CTP或两者，该酶都能很好地催化聚合物合成。根据沉降速度以及掺入聚（A）-聚（U）中的[3H]AMP与[γ-32P]ATP的摩尔比估计，合成的聚（A）-聚（U）的链长约为一百个碱基对。该反应依赖于酶浓度的平方，并且在反应过程中酶二聚体与聚（A）-聚（U）形成复合物。
