Colin P, Sirois G, Chakrabarti S
J Chromatogr. 1987 Jan 23;413:151-60. doi: 10.1016/0378-4347(87)80222-0.
The quantitation of a hepatorenal toxic drug, acetaminophen, in blood and target organ tissues is needed for toxicokinetic and distribution studies. A rapid, sensitive and simple method is described to assay acetaminophen in rat serum and liver or kidney homogenates by reversed-phase high-performance liquid chromatography, using an octadecyl (3 micron particle size) Apex column, a mobile phase consisting of a mixture of distilled water-acetonitrile (86:14) and ultraviolet detection at 245 nm. Short retention times of ca. 3.75 and 6.25 min are observed for acetaminophen and the internal standard (sulfamerazine), respectively. A sensitivity of 50 ng/ml is easily achieved for 100-microliter serum and liver or kidney homogenate samples. The proposed method proved to have satisfactory recovery, precision and accuracy. The preliminary results obtained with human plasma of volunteers and of patients treated with various drugs show that the assay, with a sensitivity of 25 ng/ml, would be of considerable interest in clinical monitoring of acetaminophen.
在毒代动力学和分布研究中，需要对肝肾毒性药物对乙酰氨基酚在血液和靶器官组织中的含量进行定量分析。本文描述了一种快速、灵敏且简便的方法，采用反相高效液相色谱法，使用十八烷基（粒径3微米）Apex柱、由蒸馏水 - 乙腈（86:14）混合物组成的流动相以及在245nm处进行紫外检测，来测定大鼠血清、肝脏或肾脏匀浆中的对乙酰氨基酚。对乙酰氨基酚和内标（磺胺嘧啶）的保留时间分别约为3.75分钟和6.25分钟。对于100微升血清以及肝脏或肾脏匀浆样品，很容易实现50纳克/毫升的灵敏度。所提出的方法具有令人满意的回收率、精密度和准确度。对服用各种药物的志愿者和患者的人血浆进行初步检测的结果表明，该检测方法灵敏度为25纳克/毫升，在对乙酰氨基酚的临床监测中具有重要意义。
