采用高效液相色谱法分析血浆和尿液中的舒林酸及其代谢产物。

Analysis of sulindac and metabolites in plasma and urine by high-performance liquid chromatography.

作者信息

Stubbs R J, Ng L L, Entwistle L A, Bayne W F

出版信息

J Chromatogr. 1987 Jan 23;413:171-80. doi: 10.1016/0378-4347(87)80224-4.

Abstract

A reversed-phase high-performance liquid chromatographic method with ultraviolet detection is described for the quantification of sulindac, sulindac sulfone and sulindac sulfide in plasma and sulindac, trans-sulindac, sulindac sulfone and sulindac sulfide in urine. Plasma samples are de-proteinized with acetonitrile and urine samples are injected directly following enzymatic hydrolysis of glucuronide metabolites. The resulting chromatograms are essentially free from endogenous interference and the limits of detection are 0.1 microgram/ml for plasma and 0.2 microgram/ml for urine for all of the above compounds.

摘要

本文描述了一种采用紫外检测的反相高效液相色谱法，用于定量测定血浆中的舒林酸、舒林酸砜和舒林酸硫化物以及尿液中的舒林酸、反式舒林酸、舒林酸砜和舒林酸硫化物。血浆样品用乙腈进行脱蛋白处理，尿液样品在对葡糖醛酸代谢物进行酶水解后直接进样。所得色谱图基本不受内源性干扰，上述所有化合物在血浆中的检测限为0.1微克/毫升，在尿液中的检测限为0.2微克/毫升。