采用高效液相色谱法测定人血浆和尿液中舒林酸及其代谢物。

Measurement of sulindac and its metabolites in human plasma and urine by high-performance liquid chromatography.

作者信息

Swanson B N, Boppana V K

出版信息

J Chromatogr. 1981 Sep 11;225(1):123-30. doi: 10.1016/s0378-4347(00)80251-0.

Abstract

A sensitive high-performance liquid chromatography assay for simultaneous measurement of sulindac and its major metabolites was developed. The extraction methods provided greater than 89% recovery of sulindac and its sulfone and sulfide metabolites from both plasma and urine. Complete resolution and accurate detection of the three compounds was achieved with a reversed-phase column, UV detection at 254 nm and a methanol-acetate buffer mobile phase. Levels of sulindac and its metabolites were determined in plasma and urine from four volunteers after oral administration of 200 mg Clinoril. Glucuronide conjugates in urine were measured after alkaline hydrolysis.

摘要

开发了一种灵敏的高效液相色谱法，用于同时测定舒林酸及其主要代谢物。该提取方法从血浆和尿液中回收舒林酸及其砜和硫化物代谢物的回收率均大于89%。使用反相柱、254 nm紫外检测和甲醇 - 醋酸盐缓冲流动相，实现了这三种化合物的完全分离和准确检测。四名志愿者口服200 mg 奇诺力后，测定了其血浆和尿液中舒林酸及其代谢物的水平。碱性水解后测定尿液中的葡萄糖醛酸结合物。