Clark C R, McMillian C L, Hoke J F, Campagna K D, Ravis W R
J Chromatogr Sci. 1987 Jun;25(6):247-51. doi: 10.1093/chromsci/25.6.247.
An improved liquid chromatographic procedure is described for the quantitative determination of sulindac, sulindac sulfone, and sulindac sulfide from serum. The procedure makes use of acetonitrile extraction of the compounds of interest from acidified serum samples. Under these conditions extraction efficiencies in the 85 percent range are obtained for each of the compounds. The liquid chromatographic separation of the compounds of interest and the internal standard (indomethacin) is accomplished in an isocratic elution procedure using a nitrile (CN) stationary phase. The HPLC separation procedure is completed in less than 10 minutes, giving excellent resolution and peak shape.
本文描述了一种改进的液相色谱法，用于定量测定血清中的舒林酸、舒林酸砜和舒林酸硫化物。该方法利用乙腈从酸化的血清样品中萃取目标化合物。在这些条件下，每种化合物的萃取效率在85%左右。目标化合物和内标（吲哚美辛）的液相色谱分离是在等度洗脱过程中使用腈基（CN）固定相完成的。高效液相色谱分离过程在不到10分钟内完成，具有出色的分离度和峰形。
