Clinical Stem Cell Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, School of Life Science, Nanjing University, Nanjing, Jiangsu Province, China.
State Key Laboratory of Pharmaceutical Biotechnology and the Comprehensive Cancer Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, School of Life Science, Nanjing University, Nanjing, Jiangsu Province, China.
Stem Cell Rev Rep. 2022 Dec;18(8):2662-2682. doi: 10.1007/s12015-022-10387-z. Epub 2022 May 19.
Stem cell therapy holds great promise for the treatment of spinal cord injury (SCI), which can reverse neurodegeneration and promote tissue regeneration via its pluripotency and ability to secrete neurotrophic factors. Although various stem cell-based approaches have shown certain therapeutic effects when applied to the treatment of SCI, their clinical efficacies have been disappointing. Thus, it is an urgent need to further enhance the neurological benefits of stem cells through bioengineering strategies including genetic engineering. In this review, we summarize the progress of stem cell therapy for SCI and the prospect of genetically modified stem cells, focusing on the genome editing tools and functional molecules involved in SCI repair, trying to provide a deeper understanding of genetically modified stem cell therapy and more applicable clinical strategies for SCI repair.
干细胞治疗在治疗脊髓损伤（SCI）方面具有广阔的前景，它可以通过多能性和分泌神经营养因子的能力逆转神经退行性变并促进组织再生。尽管各种基于干细胞的方法在应用于 SCI 治疗时显示出一定的治疗效果，但它们的临床疗效却令人失望。因此，迫切需要通过基因工程等生物工程策略进一步提高干细胞的神经益处。在这篇综述中，我们总结了干细胞治疗 SCI 的进展和基因修饰干细胞的前景，重点介绍了参与 SCI 修复的基因组编辑工具和功能分子，试图为基因修饰干细胞治疗提供更深入的了解和更适用的 SCI 修复临床策略。
