Pusch H H, Leitinger S, Winter R, Urdl W
Hum Reprod. 1986 Jun;1(4):233-5. doi: 10.1093/oxfordjournals.humrep.a136391.
Laser-Doppler-spectroscopy represents a new, fast and reliable technical method for the assessment of several parameters of sperm motility including the percentage of spermatozoa which are motile, and mean spermatozoal velocity. Serial measurements of these parameters in routine andrological work permit standards for four main spermiogram diagnoses to be established. Laser-Doppler-spectroscopy was used to verify alterations in the motility of spermatozoa after capacitation in an in-vitro fertilization programme. An enhancement of 16% for total sperm motility and 36% for mean spermatozoal velocity could be observed. The results of in-vitro fertilization after various spermigram diagnoses, combined with long-term observations of mean spermatozoal velocity, may be considered as another step towards the elucidation of sperm-oocyte interactions. Comparisons between the development of embryos revealed significant differences between normo- and asthenozoospermic men versus oligozoospermic patients.
激光多普勒光谱法是一种用于评估精子活力多个参数的新型、快速且可靠的技术方法，这些参数包括活动精子的百分比以及精子平均速度。在常规男科工作中对这些参数进行连续测量，有助于建立四种主要精液分析诊断的标准。在一项体外受精计划中，激光多普勒光谱法被用于验证精子获能后活力的变化。可以观察到总精子活力提高了16%，精子平均速度提高了36%。各种精液分析诊断后的体外受精结果，结合对精子平均速度的长期观察，可被视为朝着阐明精卵相互作用迈出的又一步。胚胎发育情况的比较显示，正常精子症和弱精子症男性与少精子症患者之间存在显著差异。
