Pusch H H
University Hospital for Gynecology and Obstetrics, Graz/Austria.
Andrologia. 1987 Sep-Oct;19(5):514-27. doi: 10.1111/j.1439-0272.1987.tb01891.x.
Motility behaviour of spermatozoa in 289 ejaculates was investigated by laser-Doppler-spectroscopy in the andrology-laboratory of the University Hospital for Gynecology and Obstetrics, Graz/Austria. Conventional spermiograms, performed on the same ejaculates, permitted comparisons between estimated motility and laser-measurements, comprising the following parameters: Total sperm motility, mean sperm velocity, percentages of progressive motile spermatozoa and mean progressive velocity. In order to elucidate the importance of sperm motility for the fertilization of oocytes in vivo and in vitro, ejaculates of six different groups of patients were evaluated. In vivo: 173 patients from the andrologic outpatient service (group A); 38 patients from the andrologic outpatient service, whose wives became pregnant (group B) and 20 donors with recently proved fertility (group C). In vitro: 8 patients, whose ejaculates failed to fertilize the oocytes (group D); 39 patients with successful fertilization (group E) and 12 patients with ongoing, clinical pregnancy of their wives after ivF (group F). In addition, motility behaviour was assessed in native ejaculates 24 hours after ejaculation, as well as changes in sperm motility after capacitation. Statistical evaluations of group comparisons revealed a good correlation between estimated results and laser measurements. Mean sperm velocity and progressive sperm motility are the most selective parameters within the group differences in oligozoospermic ejaculates. Besides motility, morphology is of importance in vivo as well as in vitro. Based on the results of measurements obtained in the different groups, the following guidelines for standard values in laser-Doppler-spectroscopy are proposed: Total sperm motility greater than 53%; mean sperm velocity greater than 42 microns/sec.; progressive sperm motility greater than 34% and mean progressive velocity greater than 68 micron/sec. If these values are not reached, medical treatment is recommended.
在奥地利格拉茨市妇产科大学医院的男科实验室，采用激光多普勒光谱法对289份精液样本中精子的运动行为进行了研究。对同一批精液样本进行常规精子活力检测，以便比较预估活力与激光测量结果，测量参数包括：精子总活力、平均精子速度、前向运动精子百分比以及平均前向运动速度。为阐明精子活力在体内和体外卵母细胞受精过程中的重要性，对六组不同患者的精液样本进行了评估。体内：男科门诊的173名患者（A组）；男科门诊中妻子已怀孕的38名患者（B组）以及20名近期证实具有生育能力的供者（C组）。体外：精液未能使卵母细胞受精的8名患者（D组）；受精成功的39名患者（E组）以及体外受精后其妻子正在临床妊娠的12名患者（F组）。此外，还评估了射精24小时后新鲜精液样本中的精子运动行为以及获能后精子活力的变化。组间比较的统计学评估显示，预估结果与激光测量结果之间具有良好的相关性。在少精子症精液样本的组间差异中，平均精子速度和前向运动精子活力是最具选择性的参数。除活力外，形态学在体内和体外都很重要。根据不同组的测量结果，提出了激光多普勒光谱法的标准值指导原则：精子总活力大于53%；平均精子速度大于42微米/秒；前向运动精子活力大于34%；平均前向运动速度大于68微米/秒。如果未达到这些值，建议进行医学治疗。
