Calos M P
Nature. 1978 Aug 24;274(5673):762-5. doi: 10.1038/274762a0.
The promoter for a weakly expressed constitutive gene, the lactose repressor gene (lacI), has been sequenced, along with an 'up' promoter mutation Iq. The 10-fold enhancement in I expression found in Iq is the result of a single base change at position -35. To facilitate the sequencing, the lacI gene was cloned in a small plasmid.
一个低表达组成型基因——乳糖阻遏蛋白基因（lacI）的启动子，已与一个“上游”启动子突变Iq一起进行了测序。在Iq中发现的I表达增强10倍是由于-35位的单个碱基变化所致。为便于测序，lacI基因被克隆到一个小质粒中。
