采用串联质谱法测定细胞内抗坏血酸。
Determination of intracellular ascorbic acid using tandem mass spectrometry.
机构信息
New Industry Creation Hatchery Center (NICHe), Tohoku University, 6-6-10 Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8579, Japan.
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
出版信息
Analyst. 2022 Jun 13;147(12):2640-2643. doi: 10.1039/d1an02160e.
Ascorbic acid is involved in a variety of biological events. Nevertheless, its detailed intracellular behavior remains unexplored due to a lack of sensitive analytical methods. Here we report a method using HPLC-ESI-MS/MS achieving the lowest detection limit ever reported (1 pg), to provide mechanistic insight into the intracellular ascorbic acid physiology.
抗坏血酸参与了多种生物事件。然而，由于缺乏敏感的分析方法，其详细的细胞内行为仍未被探索。在这里，我们报告了一种使用 HPLC-ESI-MS/MS 的方法，达到了有史以来最低的检测限（1 pg），为深入了解细胞内抗坏血酸生理学提供了机制上的见解。
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