Instituto Evandro Chagas, Departamento de Hepatologia, Belém, PA, Brazil.
Instituto Evandro Chagas, Departamento de Hepatologia, Belém, PA, Brazil.
Braz J Infect Dis. 2022 May-Jun;26(3):102367. doi: 10.1016/j.bjid.2022.102367. Epub 2022 May 20.
The goal of this study was to investigate the prevalence of occult HBV infection in a reference center for the Northern Brazil from 2005 to 2015 and to identify mutations associated with occult hepatitis B. Molecular analysis was performed on 110 serum samples in which anti-HBc was the only positive serological marker. Regions of the HBV genome were amplified by polymerase chain reaction to detect HBV DNA. A prevalence of 4.1% (793/18,889) for anti-HBc alone was identified. Molecular analysis revealed a prevalence of occult HBV infection of 0.04%. HBV DNA detected were identified in individuals who underwent hemodialysis, infected with the hepatitis C virus and from area of high endemicity for HBV. Direct DNA nucleotide sequencing and phylogenetic analysis identified that genotypes A and D and mutations E164D, I195M, P217L and P120S were associated with occult HBV infection in the S gene. This study contributed with epidemiological and molecular information on Northern Brazil samples with a suggestive profile of occult HBV infection in addition to reinforcing the importance of molecular diagnosis in this type of infection.
本研究的目的是调查 2005 年至 2015 年期间来自巴西北部参考中心的隐匿性乙型肝炎病毒感染的流行情况，并确定与隐匿性乙型肝炎相关的突变。对 110 份血清样本进行了分子分析，这些样本中唯一的阳性血清学标志物是抗 HBc。通过聚合酶链反应扩增 HBV 基因组区域，以检测 HBV DNA。抗 HBc 单独的阳性率为 4.1%（793/18889）。分子分析显示隐匿性乙型肝炎病毒感染的阳性率为 0.04%。在接受血液透析的个体、感染丙型肝炎病毒的个体以及乙型肝炎病毒高度流行地区的个体中检测到 HBV DNA。直接 DNA 核苷酸测序和系统进化分析表明，S 基因中的基因型 A 和 D 以及突变 E164D、I195M、P217L 和 P120S 与隐匿性乙型肝炎病毒感染相关。本研究为巴西北部样本提供了流行病学和分子信息，提示隐匿性乙型肝炎病毒感染的模式，同时也强调了分子诊断在这种感染中的重要性。
