von Heijne G
J Mol Biol. 1986 Nov 20;192(2):287-90. doi: 10.1016/0022-2836(86)90365-7.
The net charge distribution in a region around the signal sequence cleavage site has been calculated for samples of 41 prokaryotic and 165 eukaryotic exported proteins. The results show that prokaryotic proteins in particular have a markedly higher incidence of acidic than of basic residues in this region. The possibility that a "dipolar" structure with a positive net charge difference between the N and C-terminal regions is important for signal sequence function in bacteria is suggested, and invoked to rationalize a number of known export-defective signal sequence mutations.
已针对41种原核生物和165种真核生物输出蛋白的样本，计算了信号序列切割位点周围区域的净电荷分布。结果表明，特别是原核生物蛋白在该区域中酸性残基的发生率明显高于碱性残基。这表明N端和C端区域之间具有正净电荷差异的“偶极”结构对于细菌中的信号序列功能很重要，并据此对一些已知的输出缺陷型信号序列突变作出合理的解释。
