Sasaki M, Kawaguchi K, Yamada H, Kobayashi Y, Yamamoto H
Jpn J Antibiot. 1986 Dec;39(12):3283-90.
Effect of isepamicin (HAPA-B), a new aminoglycoside antibiotic, on fertility and reproductive performance in Wistar rats was studied. The drug was injected intramuscularly to rats at a daily dose of 25, 100 or 200 mg/kg. Male rats were treated for 63 days before mating and during the mating period, and female rats were treated for 2 weeks and through the mating period to the 7th day after mating. In male rats, an inhibition of body weight gain and a development of a pale color and hypertrophy of the kidney were observed in 100 and 200 mg/kg dose-groups. In female rats, inhibition of body weight gain was observed in 100 and 200 mg/kg dose-groups and a pale color of the kidney was seen in the 200 mg/kg group. No significant differences were observed in mating and fertility performance of male and female rats between the control and treated groups. Numbers of corpora lutea, implantation sites and living fetuses in the 200 mg/kg group decreased significantly. No external, visceral and skeletal anomalies due to the treatment of HAPA-B were observed in the live fetuses. No effect dose levels of HAPA-B found in this study were 25 mg/kg on parent rats and 100 mg/kg on reproduction ability and their fetuses.
研究了新型氨基糖苷类抗生素异帕米星（HAPA - B）对Wistar大鼠生育力和生殖性能的影响。以每日25、100或200 mg/kg的剂量给大鼠肌肉注射该药物。雄性大鼠在交配前和交配期间接受63天治疗，雌性大鼠在交配前2周及整个交配期直至交配后第7天接受治疗。在雄性大鼠中，100和200 mg/kg剂量组观察到体重增加受到抑制，肾脏出现颜色变淡和肥大。在雌性大鼠中，100和200 mg/kg剂量组观察到体重增加受到抑制，200 mg/kg组可见肾脏颜色变淡。对照组和治疗组的雄性和雌性大鼠在交配和生育性能方面未观察到显著差异。200 mg/kg组的黄体数、着床点数和存活胎儿数显著减少。在存活胎儿中未观察到因HAPA - B治疗引起的外部、内脏和骨骼异常。本研究中发现HAPA - B对亲代大鼠的无效应剂量水平为25 mg/kg，对生殖能力及其胎儿的无效应剂量水平为100 mg/kg。
