College of Chemistry and Materials Science, Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, Guangdong Engineering & Technology Research Centre of Graphene-like Materials and Products, Jinan University, Guangzhou 510632, China.
Key Laboratory of Evidence Science, China University of Political Science and Law, Beijing 100088, China.
Anal Chem. 2022 Jun 7;94(22):7936-7943. doi: 10.1021/acs.analchem.2c00736. Epub 2022 May 24.
Drug abuse is seriously endangering human health and jeopardizing society. There is an urgent need for rapid, sensitive, portable, and easy-to-operate methods for the daily detection of drugs in biological matrices. However, current drug detection methods based on chromatography, spectroscopy, immunosorbent assays, etc. are limited by the requirements of high logistical instruments and laboratory. Herein, we proposed a wearable electrochemical aptasensor with high sensitivity and specificity for the direct capture and rapid detection of multiple drugs in sweat. The single aptamer and dual aptamers with different base compositions were designed to compose the aptasensor array. Molecular docking simulations demonstrated different binding affinities between bioamines and aptamers. The developed aptasensor array is shown to be sufficient to generate distinct electrochemical fingerprints for different psychoactive drugs and interfering substances by extracting variable features from electrochemical signals. Sixteen analytes in the same concentration or gradient concentrations were identified with 100% accuracy. In addition, the wearable sensor platform was demonstrated to discriminate various drugs with similar chemical structures in artificial sweat and human sweat samples. The sensor array not only provided a new rapid method for the detection of drugs but also served as a reference for developing wearable sensors for onsite and daily testing of human biochemical information.
药物滥用严重危害人类健康，危及社会。因此，迫切需要快速、灵敏、便携且易于操作的方法来日常检测生物基质中的药物。然而，目前基于色谱法、光谱法、免疫吸附测定法等的药物检测方法受到高物流仪器和实验室的要求限制。在此，我们提出了一种具有高灵敏度和特异性的可穿戴电化学适体传感器，用于直接捕获和快速检测汗液中的多种药物。设计了具有不同碱基组成的单适体和双适体来组成适体传感器阵列。分子对接模拟表明生物胺与适体之间具有不同的结合亲和力。通过从电化学信号中提取可变特征，所开发的适体传感器阵列足以产生不同精神活性药物和干扰物质的独特电化学指纹。以相同浓度或梯度浓度的 16 种分析物的识别准确率达到了 100%。此外，该可穿戴传感器平台还证明能够在人工汗液和人体汗液样本中区分具有相似化学结构的各种药物。该传感器阵列不仅为药物检测提供了一种新的快速方法，还为开发用于现场和日常测试人体生化信息的可穿戴传感器提供了参考。
