Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.
Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom.
mBio. 2022 Jun 28;13(3):e0076122. doi: 10.1128/mbio.00761-22. Epub 2022 May 24.
What do programmed cell death (PCD) and carbohydrate metabolism by-product transport have in common? Intriguingly, both processes involve the and operons in the major human pathogen Staphylococcus aureus. Previously, CidA and LrgA have been studied in the context of programmed cell death, but a second function in overflow metabolism is increasingly evident. New work from J. L. Endres, S. S. Chaudhari, X. Zhang, J. Prahlad, et al. (mBio 13:e02827-21, 2022, https://doi.org/10.1128/mBio.02827-21) combining a lysis cassette, mutagenesis, and classic microbiology demonstrates that CidA and LrgA function as holins to support endolysin-induced lysis. But that's not all-the operon also facilitates pyruvate uptake during microaerobic and anaerobic growth. This commentary highlights the main findings from this work and places them in context of the literature to date. Finally, as these proteins are highly conserved and carry out disparate functions of great importance, it is tempting to speculate future work will elucidate the link between S. aureus lysis and pyruvate metabolism.
程序性细胞死亡 (PCD) 和碳水化合物代谢副产物转运有什么共同之处？有趣的是，这两个过程都涉及到主要人类病原体金黄色葡萄球菌中的 和 操纵子。以前，CidA 和 LrgA 已经在程序性细胞死亡的背景下进行了研究，但在溢出代谢中存在第二个功能的证据越来越多。J. L. Endres、S. S. Chaudhari、X. Zhang、J. Prahlad 等人的新工作（mBio 13:e02827-21，2022，https://doi.org/10.1128/mBio.02827-21）结合了一个裂解盒、诱变和经典微生物学，证明 CidA 和 LrgA 作为 holin 起作用，以支持内溶素诱导的裂解。但这还不是全部- 操纵子还促进了微需氧和厌氧生长过程中的丙酮酸摄取。这篇评论突出了这项工作的主要发现，并将其置于迄今为止的文献背景下。最后，由于这些蛋白质高度保守并执行具有重要意义的不同功能，因此人们不禁推测未来的工作将阐明金黄色葡萄球菌裂解和丙酮酸代谢之间的联系。
