Sakai Shinji, Taya Masahito
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
ACS Macro Lett. 2014 Oct 21;3(10):972-975. doi: 10.1021/mz5004322. Epub 2014 Sep 17.
Hydrogel sheaths were fabricated on the surfaces of individual mammalian cells through the cross-linking of polymer molecules catalyzed by horseradish peroxidase (HRP) in aqueous solution. For confining the progress of the cross-linking only on the cell surface, HRP was anchored to the cell membrane by soaking the cells in the solution containing the HRP conjugated with a biocompatible anchor molecule for cell membrane. The hydrogel sheath of about 1 μm thickness was obtained by soaking the cells with the anchored HRP in aqueous solution containing polymers possessing phenolic hydroxyl (Ph) moieties and HO for 10 min. The hydrogel sheaths could be made from a variety of polymers possessing Ph moieties, for example, derivatives of polysaccharide, protein, and synthetic polymer. Cytocompatibility of the on-cell surface enzymatic hydrogel sheath formation was confirmed from the viability of the enclosed cells (>90%) and subsequent normal growth after removal of the hydrogel sheath.
通过辣根过氧化物酶（HRP）在水溶液中催化聚合物分子交联，在单个哺乳动物细胞表面制备了水凝胶鞘。为了将交联过程限制在细胞表面，通过将细胞浸泡在含有与细胞膜生物相容性锚定分子偶联的HRP的溶液中，使HRP锚定在细胞膜上。通过将带有锚定HRP的细胞浸泡在含有具有酚羟基（Ph）部分的聚合物和HO的水溶液中10分钟，获得了厚度约为1μm的水凝胶鞘。水凝胶鞘可以由多种具有Ph部分的聚合物制成，例如多糖、蛋白质和合成聚合物的衍生物。从被包裹细胞的活力（>90%）以及去除水凝胶鞘后的正常生长情况，证实了细胞表面酶促水凝胶鞘形成的细胞相容性。
