Ando J, Nomura H, Kamiya A
Microvasc Res. 1987 Jan;33(1):62-70. doi: 10.1016/0026-2862(87)90007-0.
We have examined the effect of shear stress on the regenerative response of cultured vascular endothelial cells by using a fluid shear apparatus designed in our laboratory. The shear stress was created on the endothelial cell layer of a fetal calf and grown confluently in a culture dish by whirling the medium, with a rotating disk placed on the fluid surface. The effect of the shear load (0.3-1.7 dyn/cm2) over 24 hr was evaluated by counting the number of regenerated cells in a denuded area that had been created by mechanically removing some cells before rotating the medium. The cell number observed in the denuded area after the exposure to shear stress was about twice as great as that of the static control. The difference was statistically significant (P less than 0.01 to P less than 0.05). Cell migration and proliferation occurred more prominently in the downstream portion of the flow than in the upstream part. The cell number in the downstream portions correlated significantly with the intensity of the applied shear stress (P less than 0.05). These results indicate that shear stress can stimulate the migration and proliferation of endothelial cells.
我们使用在我们实验室设计的流体剪切装置，研究了剪切应力对培养的血管内皮细胞再生反应的影响。通过旋转放置在流体表面的圆盘来旋转培养基，从而在胎牛内皮细胞层上产生剪切应力，该细胞层在培养皿中汇合生长。通过计算在旋转培养基之前通过机械去除一些细胞而形成的裸露区域中再生细胞的数量，评估了24小时内剪切负荷（0.3-1.7达因/平方厘米）的影响。暴露于剪切应力后在裸露区域观察到的细胞数量约为静态对照的两倍。差异具有统计学意义（P小于0.01至P小于0.05）。细胞迁移和增殖在流动的下游部分比在上游部分更明显。下游部分的细胞数量与施加的剪切应力强度显著相关（P小于0.05）。这些结果表明，剪切应力可以刺激内皮细胞的迁移和增殖。
