Department of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.
Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, USA.
J R Soc Interface. 2021 Oct;18(183):20210752. doi: 10.1098/rsif.2021.0752. Epub 2021 Oct 27.
The progression of cardiac gene expression-wall shear stress (WSS) interplay is critical to identifying developmental defects during cardiovascular morphogenesis. However, mechano-genetics from the embryonic to larval stages are poorly understood in vertebrates. We quantified peak WSS in the heart and tail vessels of Japanese medaka from 3 days post fertilization (dpf) to 14 dpf using micro-particle image velocimetry flow measurements, and in parallel analysed the expression of five cardiac genes (, , , , ). Here, we report that WSS in the atrioventricular canal (AVC), ventricular outflow tract (OFT), and the caudal vessels in medaka peak with inflection points at 6 dpf and 10-11 dpf instead of a monotonic trend. Retrograde flows are captured at the AVC and OFT of the medaka heart for the first time. In addition, all genes were upregulated at 3 dpf and 7 dpf, indicating a possible correlation between the two, with the cardiac gene upregulation preceding WSS increase in order to facilitate cardiac wall remodelling.
心脏基因表达与壁面切应力（WSS）相互作用的进展对于确定心血管发生过程中的发育缺陷至关重要。然而，脊椎动物从胚胎到幼虫阶段的力遗传学仍知之甚少。我们使用微粒子图像测速流测量法，量化了从受精后 3 天（dpf）到 14 dpf 的日本青鳉心脏和尾部血管中的峰值 WSS，并同时分析了五个心脏基因（、、、、）的表达。在这里，我们报告说，青鳉的房室管（AVC）、心室流出道（OFT）和尾部血管中的 WSS 在 6 dpf 和 10-11 dpf 出现拐点，而不是单调趋势。青鳉心脏的 AVC 和 OFT 首次捕获到逆行流。此外，所有基因在 3 dpf 和 7 dpf 时都上调，表明两者之间可能存在相关性，心脏基因的上调先于 WSS 的增加，以促进心脏壁的重塑。
