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空肠弯曲菌分离方法的比较及水分含量对菌落形态的影响

[Comparison of Campylobacter jejuni isolation methods and the effect of moisture content on colony morphology].

作者信息

Diker K S, Yardimci H

出版信息

Mikrobiyol Bul. 1986 Jul;20(3):115-9.

PMID:3561277
Abstract

Several isolation methods and media and the effect of moisture content on colony morphology were compared for the primary isolation of Campylobacter jejuni. Of the 200 rectal swabs from cattle and sheep tested for the isolation of C. jejuni, 19.5% were positive on Butzler medium and 16.5% were positive on Skirrow medium. The transportation of samples in modified Cary-Blair medium increased the isolation rate. Of the 300 gallbladder from cattle and sheep tested for the isolation of C. jejuni, 5% were positive by direct inoculation of bile, 27% were positive by swabbing and 31% were positive by selective enrichment method. The organism produced two different type of colonies on fresh and dried media.

摘要

为了对空肠弯曲菌进行初次分离,比较了几种分离方法、培养基以及水分含量对菌落形态的影响。在检测空肠弯曲菌分离情况的200份牛羊直肠拭子中,19.5%在布茨勒培养基上呈阳性,16.5%在斯基罗培养基上呈阳性。在改良的卡里-布莱尔培养基中运送样本提高了分离率。在检测空肠弯曲菌分离情况的300份牛羊胆囊中,直接接种胆汁5%呈阳性,擦拭取样27%呈阳性,选择性富集法31%呈阳性。该菌在新鲜培养基和干燥培养基上产生两种不同类型的菌落。

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