Patton C M, Mitchell S W, Potter M E, Kaufmann A F
J Clin Microbiol. 1981 Feb;13(2):326-30. doi: 10.1128/jcm.13.2.326-330.1981.
Three selective media, Skirrow, Butzler, and a modification of Butzler medium, were compared for the primary isolation of Campylobacter fetus subsp. jejuni. This organism was isolated from 87 of 347 specimens (72 from 240 dogs rectal swabs and 15 from 107 cats rectal swabs). The positive rate for dogs (30%) was twice as high as that for cats (14%). Skirrow and Butzler media were comparable in their isolation of C. fetus subsp. jejuni. A significantly higher rate of positive results was obtained with modified Butzler medium. The best combination of two media was that of modified Butzler and Skirrow media, which detected 98% of the isolates obtained. The percentage of Campylobacter-positive specimens was increased by 9% by holding primary isolation plates 72 h.
比较了三种选择性培养基,即斯基罗培养基、布茨勒培养基以及布茨勒培养基的改良培养基,用于胎儿弯曲菌空肠亚种的初次分离。从347份标本中的87份分离出了该菌(240份犬直肠拭子中的72份以及107份猫直肠拭子中的15份)。犬的阳性率(30%)是猫(14%)的两倍。斯基罗培养基和布茨勒培养基在分离胎儿弯曲菌空肠亚种方面效果相当。改良布茨勒培养基获得的阳性结果率显著更高。两种培养基的最佳组合是改良布茨勒培养基和斯基罗培养基,它们检测出了98%的分离菌株。将初次分离平板放置72小时,弯曲菌阳性标本的百分比增加了9%。