von der Ehe Christian, Buś Tanja, Weber Christine, Stumpf Steffi, Bellstedt Peter, Hartlieb Matthias, Schubert Ulrich S, Gottschaldt Michael
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743 Jena, Germany.
Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany.
ACS Macro Lett. 2016 Mar 15;5(3):326-331. doi: 10.1021/acsmacrolett.5b00856. Epub 2016 Feb 15.
A highly porous cryogel is prepared and subsequently functionalized with an atom transfer radical polymerization (ATRP) initiator at the surface. Two new glycomonomers are introduced, which possess deprotected mannose as well as glucose moieties. These are copolymerized with -propylacrylamide (iPAm) from the cryogel surface, providing a highly hydrophilic porous material, which is characterized by SEM, FT-IR spectroscopy, and NMR spectroscopy. This functionalized support can be applied for affinity chromatography of whole cells owing to the high pore space and diameter. Such an application is exemplified by investigating the ability to capture bacteria, revealing selective binding interactions of the bacteria with the mannose glycopolymer-functionalized cryogel surface. Thus, the presented glycopolymer-cryogel represents a promising material for affinity chromatography or enrichment of cells.
制备了一种高度多孔的冷冻凝胶，随后在其表面用原子转移自由基聚合（ATRP）引发剂进行功能化处理。引入了两种新的糖单体，它们具有脱保护的甘露糖以及葡萄糖部分。这些糖单体与冷冻凝胶表面的异丙基丙烯酰胺（iPAm）共聚，得到一种高度亲水的多孔材料，通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和核磁共振光谱（NMR）对其进行了表征。由于具有高孔隙空间和直径，这种功能化载体可用于全细胞的亲和色谱。通过研究捕获细菌的能力来举例说明这种应用，揭示了细菌与甘露糖糖聚合物功能化冷冻凝胶表面的选择性结合相互作用。因此，所呈现的糖聚合物-冷冻凝胶是用于亲和色谱或细胞富集的一种有前景的材料。
