Ogata Yutaro, Seto Hirokazu, Murakami Tatsuya, Hoshino Yu, Miura Yoshiko
Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Membranes (Basel). 2013 Jul 30;3(3):169-81. doi: 10.3390/membranes3030169.
Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measurements. The interaction between lectin and the glycopolymer immobilized on glass slides was confirmed using fluorescent-labeled proteins. Glycopolymer-immobilized surfaces exhibited specific adsorption of the corresponding lectin, compared with bovine serum albumin. Lectins were continuously rejected by the glycopolymer-immobilized membranes. When the protein solution was permeated through the glycopolymer-immobilized membrane, bovine serum albumin was not adsorbed on the membrane surface. In contrast, concanavalin A and wheat germ agglutinin were rejected by membranes incorporating D-mannose or N-acetyl-D-glucosamine, respectively. The amounts of adsorbed concanavalin A and wheat germ agglutinin was increased five- and two-fold that of adsorbed bovine serum albumin, respectively.
制备了带有糖聚合物刷的多孔膜作为用于亲和分离的生物材料。糖聚合物刷含有丙烯酸和D-甘露糖或N-乙酰-D-葡萄糖胺，并通过表面引发的原子转移自由基聚合在基底上形成。通过X射线光电子能谱、接触角和椭偏测量证实了糖聚合物刷的存在。使用荧光标记蛋白证实了凝集素与固定在载玻片上的糖聚合物之间的相互作用。与牛血清白蛋白相比，固定有糖聚合物的表面表现出对相应凝集素的特异性吸附。凝集素被固定有糖聚合物的膜持续排斥。当蛋白质溶液透过固定有糖聚合物的膜时，牛血清白蛋白未吸附在膜表面。相反，伴刀豆球蛋白A和麦胚凝集素分别被掺入D-甘露糖或N-乙酰-D-葡萄糖胺的膜排斥。吸附的伴刀豆球蛋白A和麦胚凝集素的量分别是吸附的牛血清白蛋白量的五倍和两倍。
