Lu Ping, Ji Xuemeng, Xue Juan, Dong Yinping, Chen Xi
Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Institute, Tianjin Eye Hospital, Tianjin, China.
Nankai University Affiliated Eye Hospital, Nankai University, Tianjin, China.
Front Microbiol. 2022 May 9;13:888103. doi: 10.3389/fmicb.2022.888103. eCollection 2022.
Screening drugs and compounds to fight against (), one of the most common pathogens that can cause fatal necrotizing enterocolitis, septicema and meningitis, is still needed. We found that -coumaric acid (pCA) has an inhibitory effect on and . Proteomic changes of BAA-894 exposed to pCA were studied to reveal the antibacterial mechanisms involved. A total of 1,553 proteins were identified in BAA-894 by label-free proteomics analysis. Fuzzy cluster analysis showed that 33 were up-regulated, and 110 were down-regulated with pCA treatment. Gene Ontology (GO) analysis concluded that pCA caused the change of metabolic state of bacteria and generally in the state of metabolic inhibition. KEGG Enrichment Analysis (KEGG) analysis showed that pCA inhibited energy metabolism and distorted the balance of amino acid metabolism. Posttranslational modification analysis showed that pCA affected the deamidation of three proteins, including Elongation factor Tu, one of the vital proteins in bacteria. Molecular docking suggested the hydrogen bond between the pCA carboxyl group and Elongation factor Tu Asn-64 might contribute to deamidation. Overall, we found that pCA interfered with cellular energy and amino acid metabolism and promoted elongation factor Tu deamidation, suggesting that pCA can be an effective natural substitute to control .
筛选用于对抗（）的药物和化合物仍然是必要的，（）是最常见的病原体之一，可导致致命的坏死性小肠结肠炎、败血症和脑膜炎。我们发现对香豆酸（pCA）对（）和（）具有抑制作用。研究了暴露于pCA的（）BAA - 894的蛋白质组变化，以揭示其涉及的抗菌机制。通过无标记蛋白质组学分析在（）BAA - 894中总共鉴定出1553种蛋白质。模糊聚类分析表明，经pCA处理后，有33种蛋白质上调，110种蛋白质下调。基因本体（GO）分析得出结论，pCA导致细菌代谢状态发生变化，总体处于代谢抑制状态。京都基因与基因组百科全书（KEGG）富集分析表明，pCA抑制能量代谢并破坏氨基酸代谢平衡。翻译后修饰分析表明，pCA影响三种蛋白质的脱酰胺作用，包括细菌中重要蛋白质之一的延伸因子Tu。分子对接表明，pCA羧基与延伸因子Tu的Asn - 64之间的氢键可能有助于脱酰胺作用。总体而言，我们发现pCA干扰细胞能量和氨基酸代谢并促进延伸因子Tu脱酰胺作用，这表明pCA可以成为控制（）的有效天然替代品。
