College of Chemistry, Jilin University, Changchun, 130012, China.
College of Chemistry, Jilin University, Changchun, 130012, China; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Sciences, Jilin University, Changchun, 130012, China.
Talanta. 2022 Sep 1;247:123563. doi: 10.1016/j.talanta.2022.123563. Epub 2022 May 19.
When applied as adsorbents for phosphopeptides enrichment, two-dimensional (2D) layered double hydroxides (LDHs) are usually limited by the disadvantages of buried affinity sites and reduced specific surface area. Multifarious exfoliation strategies have been implemented to compensate for these deficiencies, but tedious exfoliation process cannot meet the requirements of LDHs as high-efficiency adsorbents. Incorporating LDHs with three-dimensional (3D) template can avoid tedious exfoliation and produce hierarchical LDHs with large specific surface area and massive affinity sites. Herein, a hierarchical LDH (denoted as FeO@ZIF-8@Zn-Ga LDH) was prepared by metal-organic framework (MOF)-mediated synthesis strategy, and a magnetic solid-phase extraction (MSPE) platform was constructed and employed for phosphopeptides enrichment with high efficiency. The unique 3D structure and abundant metal nodes of MOF provide 3D template and metal sources for in-situ nucleation and generation of LDH. Large specific surface area and massive exposed Zn and Ga endow FeO@ZIF-8@Zn-Ga LDH with high enrichment efficiency toward phosphopeptides from complicated biological samples. With the aid of mass spectrometry (MS) techniques, we profiled endogenous or global phosphopeptides from human saliva and serum, which proved the practical application value of this material.
当用作磷肽富集的吸附剂时，二维（2D）层状双氢氧化物（LDHs）通常受到埋藏亲和位点和减少的比表面积的缺点的限制。已经实施了多种剥离策略来弥补这些缺陷，但繁琐的剥离过程不能满足作为高效吸附剂的 LDHs 的要求。将 LDHs 与三维（3D）模板结合可以避免繁琐的剥离，并产生具有大比表面积和大量亲和位点的分层 LDHs。在此，通过金属有机骨架（MOF）介导的合成策略制备了分层 LDH（表示为 FeO@ZIF-8@Zn-Ga LDH），并构建了磁性固相萃取（MSPE）平台，用于高效富集磷肽。MOF 的独特 3D 结构和丰富的金属节点为 LDH 的原位成核和生成提供了 3D 模板和金属源。大的比表面积和大量暴露的 Zn 和 Ga 使 FeO@ZIF-8@Zn-Ga LDH 对来自复杂生物样品的磷肽具有高富集效率。借助质谱（MS）技术，我们从人唾液和血清中分析了内源性或全磷肽，证明了该材料的实际应用价值。
