Department of Orthopedics, Changhai hospital Affiliated to the Navy Military Medical University, Shanghai, China.
Department of Orthopedics, Seventh medical center of PLA general hospital, Beijing, China.
Colloids Surf B Biointerfaces. 2022 Aug;216:112582. doi: 10.1016/j.colsurfb.2022.112582. Epub 2022 May 18.
Titanium nanotube (Ti-NT) is an attractive substrate for local drug delivery, however, it is difficult to control the burst drug release and achieve sustained release from these nanotubes. In the present study, we investigated the feasibility of controlling drug release from Ti-NT within polydopamine and hyaluronic acid films, to achieve antibacterial activity and osteogenic promotion. Vancomycin was loaded into the Ti-NT by lyophilisation. Dopamine and hyaluronic acid were immobilized on the vancomycin-loaded Ti-NT surface through alternate deposition technique. The anti-infective and osteogenic abilities of the polydopamine and hyaluronic acid-modified Ti-NT were then investigated. Our results demonstrated that polydopamine and hyaluronic acid-modified Ti-NT exhibited improved drug loading and release control for 7 days. Compared with the vancomycin-loaded Ti-NT, the polydopamine and hyaluronic acid-modified Ti-NT exhibited better antibacterial ability, and the hyaluronic acid-modified Ti-NT promoted the osteogenic differentiation of rat bone marrow stem cells. Our results demonstrated that Ti-NT biofunctionalized with polydopamine and hyaluronic acid can help overcome the limitations of Ti-NT, by improving drug loading, antibacterial activity and osteogenic ability.
钛纳米管（Ti-NT）是局部药物输送的有吸引力的基底，然而，很难控制这些纳米管中药物的突释，并实现持续释放。在本研究中，我们研究了在聚多巴胺和透明质酸薄膜内控制 Ti-NT 中药物释放的可行性，以实现抗菌活性和促进成骨。通过冻干法将万古霉素载入 Ti-NT。通过交替沉积技术将多巴胺和透明质酸固定在载万古霉素的 Ti-NT 表面。然后研究了聚多巴胺和透明质酸修饰的 Ti-NT 的抗感染和促成骨能力。我们的结果表明，聚多巴胺和透明质酸修饰的 Ti-NT 表现出改善的药物负载和 7 天的释放控制。与载万古霉素的 Ti-NT 相比，聚多巴胺和透明质酸修饰的 Ti-NT 表现出更好的抗菌能力，并且透明质酸修饰的 Ti-NT 促进了大鼠骨髓间充质干细胞的成骨分化。我们的结果表明，用聚多巴胺和透明质酸生物功能化的 Ti-NT 可以通过提高药物负载、抗菌活性和成骨能力来帮助克服 Ti-NT 的局限性。
