Bio Artificial Organ and Regenerative Medicine Unit, National Defense University of Malaysia, Sungai Besi Camp, 57000, Kuala Lumpur, Malaysia.
Stem Cell & Immunity Research Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400, Serdang, Malaysia.
Sci Rep. 2022 May 26;12(1):8904. doi: 10.1038/s41598-022-12653-z.
Despite the extensive reports on the potential hazard of magnetic field (MF) exposures on humans, there are also concurrently reported on the improved proliferative property of stem cells at optimum exposure. However, the effect on mesenchymal stem cells (MSCs) remains unknown. Therefore, we aimed to investigate the impact of induced static MF (SMF) on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) using Samarium Cobalt (SmCO5). At passage 3, hUC-MSCs (1 × 10) were exposed to 21.6 mT SMF by a direct exposure (DE) showed a significantly higher cell count (p < 0.05) in the growth kinetics assays with the shortest population doubling time relative to indirect exposure and negative control. The DE group was committed into the cell cycle with increased S phase (55.18 ± 1.38%) and G2/M phase (21.75 ± 1.38%) relative to the NC group [S-phase (13.54 ± 2.73%); G2/M phase (8.36 ± 0.28%)]. Although no significant changes were observed in the immunophenotype, the DE group showed an elevated expression of pluripotency-associated markers (OCT4, SOX2, NANOG, and REX1). These results suggest that the MFs could potentially induce proliferation of MSCs, a promising approach to promote stem cells propagation for clinical therapy and research without compromising the stemness of hUC-MSCs.
尽管有大量关于磁场 (MF) 暴露对人类潜在危害的报道，但也有报道称，在最佳暴露下，干细胞的增殖特性得到了改善。然而，其对间充质干细胞 (MSCs) 的影响尚不清楚。因此，我们旨在使用钐钴 (SmCO5) 研究诱导静磁场 (SMF) 对人脐带间充质干细胞 (hUC-MSCs) 的影响。在第 3 代时，通过直接暴露 (DE) 将 1×10 的 hUC-MSCs 暴露于 21.6 mT SMF 中，与间接暴露和阴性对照相比，生长动力学检测显示细胞计数显著增加 (p<0.05)，倍增时间最短。DE 组细胞周期进程加快，S 期（55.18±1.38%）和 G2/M 期（21.75±1.38%）比例增加，而 NC 组 S 期（13.54±2.73%）和 G2/M 期（8.36±0.28%）比例减少。虽然免疫表型没有明显变化，但 DE 组显示多能性相关标志物（OCT4、SOX2、NANOG 和 REX1）的表达水平升高。这些结果表明，MF 可能潜在地诱导 MSC 增殖，这是一种有前途的促进干细胞增殖的方法，可用于临床治疗和研究，而不会损害 hUC-MSCs 的干性。
