Marędziak Monika, Tomaszewski Krzysztof, Polinceusz Paulina, Lewandowski Daniel, Marycz Krzysztof
a Faculty of Veterinary Medicine , University of Environmental and Life Sciences , Wrocław , Poland.
b Faculty of Biology , University of Environmental and Life Sciences , Wrocław , Poland.
Electromagn Biol Med. 2017;36(1):45-54. doi: 10.3109/15368378.2016.1149860. Epub 2016 Jul 1.
The aim of this work was to investigate the effects of 0.5T static magnetic field (sMF) on the viability and proliferation rate of human adipose-derived mesenchymal stromal stem cells (hASCs) via activation of the phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathway. In a 7-d culture we examined cell growth kinetic and population doubling time (PDT). We also examined cell morphology and the cellular senescence markers level. Exposure to sMF enhanced the viability of these cells. However, the effect was blocked by treating the cells with LY294002, a P13K inhibitor. We compared this effect by Western Blot analysis of Akt protein expression. We also examined whether the cell response on sMF stimulation is dependent on integrin engagement and we measured integrin gene expression. Our results suggest that stimulation using sMF is a viable method to improve hASC viability. sMF is involved in mechanisms associated with controlling cell proliferative potential signaling events.
本研究旨在通过激活磷酸肌醇3激酶/蛋白激酶B（PI3K/Akt）信号通路，探讨0.5T静磁场（sMF）对人脂肪间充质干细胞（hASC）活力和增殖率的影响。在7天的培养过程中，我们检测了细胞生长动力学和群体倍增时间（PDT）。我们还检测了细胞形态和细胞衰老标志物水平。暴露于sMF可提高这些细胞的活力。然而，用PI3K抑制剂LY294002处理细胞可阻断该效应。我们通过蛋白质免疫印迹分析Akt蛋白表达来比较这种效应。我们还检测了细胞对sMF刺激的反应是否依赖于整合素的参与，并测量了整合素基因表达。我们的结果表明，使用sMF刺激是提高hASC活力的一种可行方法。sMF参与了与控制细胞增殖潜能信号事件相关的机制。
