Sadri Maryam, Abdolmaleki Parviz, Abrun Saeid, Beiki Bahareh, Samani Fazel Sahraneshin
1Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 1415-154, Tehran, Iran.
2Department of Hematology, Faculty of Biological Sciences, Tarbiat Modares University (TMU), P.O. Box 14115-175, Tehran, Iran.
Cell Mol Bioeng. 2017 Mar 20;10(3):249-262. doi: 10.1007/s12195-017-0482-y. eCollection 2017 Jun.
This investigation is performed to evaluate the impact of static magnetic field on the Cell growth alignment, and differentiation potential in Human Mesenchymal Stem cells derived from human newborn cords. -cultured mesenchymal stem cells derived from human newborn cords were exposed to SMF up to 24 mT and compared with the control (unexposed) cultures. Viability was assessed Trypan Blue staining and MTT assay. Cell cycle progression was studied after flow cytometry data analysis. Sox-2, Nanong, and Oct-4 Primers used for RT-PCR experiment. Morphological studies showed that the exposed cells were significantly aligned in parallel bundles in a correlation with the magnetic field lines. Viability measurements showed a significant reduction in cell viability which was noted after exposure to static magnetic field and initiated 36 h after the end of exposure time. Flow cytometric data analysis confirmed a decrease in G1 phase cell population within the treated and cultured groups compared with the corresponding control samples. However, the induced changes were recovered in the cell cultures after the post-exposure culture recovery time which may be attributed to the cellular repair mechanisms. Furthermore, the proliferation rate and Oct-4 gene expression were reduced due to the 18 mT static magnetic field exposure. The significant proliferation rate decrease accompanied by the Sox-2, Nanong, and Oct-4 gene expression decline, suggested the differentiation inducing effects of SMF exposure. Exposure to Static Magnetic fields up to 24 mT affects mesenchymal stem cell alignment and proliferation rate as well as mRNA expression of Sox-2, Nanong, and Oct-4 genes, therefore can be considered as a new differentiation inducer in addition to the other stimulators.
本研究旨在评估静磁场对源自人类新生儿脐带的人间充质干细胞的细胞生长排列和分化潜能的影响。将源自人类新生儿脐带的培养间充质干细胞暴露于高达24 mT的静磁场中，并与对照（未暴露）培养物进行比较。通过台盼蓝染色和MTT试验评估细胞活力。在流式细胞术数据分析后研究细胞周期进程。使用Sox-2、Nanong和Oct-4引物进行RT-PCR实验。形态学研究表明，暴露的细胞与磁场线相关，显著平行排列成束。活力测量显示，暴露于静磁场后细胞活力显著降低，且在暴露时间结束后36小时开始出现。流式细胞术数据分析证实，与相应的对照样品相比，处理和培养组中G1期细胞群体减少。然而，在暴露后培养恢复时间后，细胞培养物中的诱导变化得以恢复，这可能归因于细胞修复机制。此外，由于暴露于18 mT静磁场，增殖率和Oct-4基因表达降低。显著的增殖率下降伴随着Sox-2、Nanong和Oct-4基因表达的下降，表明静磁场暴露具有分化诱导作用。暴露于高达24 mT的静磁场会影响间充质干细胞的排列和增殖率以及Sox-2、Nanong和Oct-4基因的mRNA表达，因此除其他刺激物外，可被视为一种新的分化诱导剂。
