Ma Jing, Dong Suhe, Lu Hongtao, Chen Zhongmin, Yu Huijie, Sun Xuejun, Peng Renjun, Li Wei, Wang Sinian, Jiang Qisheng, Li Fengsheng, Ma Li
The Postgraduate Training Base of Jinzhou Medical University (The PLA Rocket Force Characteristic Medical Center), Beijing, 100088, China.
PLA Rocket Force Characteristic Medical Center, Beijing, 100088, China.
Biomater Res. 2022 May 26;26(1):20. doi: 10.1186/s40824-022-00266-6.
This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH on radiation-induced male fertility impairment.
The characterization of MgH were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH were evaluated in vivo and in vitro. The radioprotective effect of MgH on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms.
MgH had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH was safe and nontoxic in mice and cells. After irradiation, MgH treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH inhibited the activation of radiation-induced inflammation and pyroptosis.
MgH improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals. Mice fertility and function were evaluated with or without MgH treatment after 5 Gy irradiation. MgH had the ability of hydroxyl radicals scavenging and MDA suppressing in testicular tissue induced by irradiation. Further, MgH could participate in spermatogenesis and protect sperm development in three stages: the generation of Sertoli cells (Sox-9+), spermatogonia (Stra8+) and round sperm cells (Crem+). Moreover, MgH alleviated the decrease of testosterone secreted by interstitial cells after irradiation. In addition, MgH suppressed apoptosis, pyroptosis and inflammatory response and alleviated cell cycle arrest by mediating IR-induced ROS.
本研究旨在揭示储氢纳米材料MgH对辐射诱导的雄性生育能力损伤的保护作用。
通过扫描电子显微镜（SEM）和粒度分析仪对MgH进行表征分析。在体内和体外评估MgH的安全性。分析MgH对小鼠生殖系统的辐射防护作用，包括精子质量、遗传效应、精子发生和激素分泌。采用电子自旋共振（ESR）、流式细胞术和蛋白质免疫印迹法揭示其潜在机制。
MgH呈不规则球形形态，粒径约为463.2 nm，镁含量达71.46%。MgH在小鼠和细胞中安全无毒。辐照后，MgH处理显著保护睾丸结构，增加精子密度，提高精子活力，降低畸形率，并降低遗传毒性。特别是，精子活力与假照射小鼠和人类精液样本中的一致。此外，MgH处理可维持激素分泌和睾丸精子发生，尤其是支持细胞、精原细胞和圆形精子细胞的生成。在体外，MgH消除了[·OH]，抑制了辐照诱导的活性氧生成增加，并有效减轻了丙二醛含量的增加。此外，MgH显著改善睾丸和细胞中的凋亡，并逆转了辐照诱导的G2/M期细胞周期阻滞。此外，MgH抑制辐射诱导的炎症和焦亡的激活。
MgH通过消除羟基自由基改善辐射诱导的雄性生育能力损伤。在5 Gy辐照后，对有或无MgH处理的小鼠生育能力和功能进行评估。MgH具有清除辐照诱导的睾丸组织中羟基自由基和抑制丙二醛的能力。此外，MgH可参与精子发生，并在支持细胞（Sox-9+）、精原细胞（Stra8+）和圆形精子细胞（Crem+）三个阶段保护精子发育。此外，MgH减轻了辐照后间质细胞分泌睾酮的减少。此外，MgH通过介导辐照诱导的活性氧抑制凋亡、焦亡和炎症反应，并减轻细胞周期阻滞。
