Cruz-Davila Jhon, Perez Jeffrey Vargas, Castillo Daynet Sosa Del, Diez Nardy
ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Biotechnology Research Center of Ecuador, CIBE, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador.
Biotechnol Rep (Amst). 2022 May 17;35:e00738. doi: 10.1016/j.btre.2022.e00738. eCollection 2022 Sep.
The xylanolytic potential of endophytic fungi isolated from leaves of was explored for the first time. Four fungal strains showed significant amounts of xylanase activity and low cellulase levels when grown on wheat bran as the sole carbon source. Strain Ec220 of had the highest xylanase production (1.79 U/ml), whereas its cellulase activity was minimal (0.24 U/ml). Optimal conditions for xylanase production were: 154 h of incubation time, pH 5.79 and 29.8 °C. Furthermore, two protein spots detected by two-dimensional gel electrophoresis showed molecular weights (26.05 and 27.70 kDa) and isoelectric points (6.18 and 9.20) corresponding to previously reported xylanases, Xyl A and Xyl B, respectively. Therefore, endophytic fungi of can be an important source of xylanolytic activities when cultured on wheat bran, and xylanases with low cellulases found in strain Ec220 require further characterization as they show promise for possible industrial applications.
首次探索了从[植物名称未给出]叶片中分离出的内生真菌的木聚糖分解潜力。当以麦麸作为唯一碳源生长时，四种真菌菌株表现出大量的木聚糖酶活性和较低的纤维素酶水平。[植物名称未给出]的Ec220菌株木聚糖酶产量最高（1.79 U/ml），而其纤维素酶活性最低（0.24 U/ml）。木聚糖酶生产的最佳条件为：培养时间154小时、pH 5.79和29.8℃。此外，二维凝胶电泳检测到的两个蛋白质斑点显示分子量（分别为26.05和27.70 kDa）和等电点（分别为6.18和9.20），分别对应于先前报道的[植物名称未给出]木聚糖酶Xyl A和Xyl B。因此，[植物名称未给出]的内生真菌在以麦麸培养时可能是木聚糖分解活性的重要来源，并且在Ec220菌株中发现的低纤维素酶木聚糖酶因其显示出可能的工业应用前景而需要进一步表征。
