Fasiku Samuel Adedayo, Bello Mobolaji Akeem, Odeniyi Olubusola Ayoola
Department of Biological Sciences, Ajayi Crowther University, Oyo Town, Oyo State, Nigeria.
Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
Access Microbiol. 2023 Jun 14;5(6). doi: 10.1099/acmi.0.000506.v5. eCollection 2023.
Xylanase breaks xylan down to xylose, which is used in industries such as pulp and paper, food and feed, among others. The utilization of wastes for xylanase production is economical, hence this work aimed at producing xylanase through solid-state fermentation and characterizing the enzyme. Xylanase-producing strains of and GIO were inoculated separately in a 5 and 10 day solid fermentation study on maize straw, rice straw, sawdust, corn cob, sugarcane bagasse, conifer litters, alkaline-pretreated maize straw (APM) and combined alkaline and biological-pretreated maize straw, respectively. The best substrate was selected for xylanase production. The crude enzyme was extracted from the fermentation medium and xylanase activity was characterized using parameters such as temperature, cations, pH and surfactants. Among different substrates, the highest xylanase activity of 3.18 U ml was recorded when GIO was grown on APM. The xylanase produced by GIO and had the highest activities (3.67 U ml and 3.36 U ml) at 40 °C after 30 and 45 min of incubation, respectively. Optimal xylanase activities (4.58 and 3.58 U ml) of GIO and , respectively, were observed at pH 5.0 and 6.2. All cations used enhanced xylanase activities except magnesium ion. Sodium dodecyl sulfate supported the highest xylanase activity of 6.13 and 6.90 U ml for GIO and , respectively. High yields of xylanase were obtained from GIO and cultivated on APM. The xylanase activities were affected by pH, temperature, surfactants and cations.
木聚糖酶将木聚糖分解为木糖，木糖用于纸浆和造纸、食品和饲料等行业。利用废弃物生产木聚糖酶具有经济性，因此这项工作旨在通过固态发酵生产木聚糖酶并对该酶进行表征。分别将产木聚糖酶的菌株和GIO接种到玉米秸秆、稻草、锯末、玉米芯、甘蔗渣、针叶树落叶、碱预处理玉米秸秆（APM）以及碱和生物联合预处理玉米秸秆上，进行为期5天和10天的固态发酵研究。选择最佳底物用于生产木聚糖酶。从发酵培养基中提取粗酶，并使用温度、阳离子、pH值和表面活性剂等参数对木聚糖酶活性进行表征。在不同底物中，当GIO在APM上生长时，木聚糖酶活性最高，达到3.18 U/ml。GIO和产生的木聚糖酶分别在40℃孵育30分钟和45分钟后活性最高（分别为3.67 U/ml和3.36 U/ml）。在pH值为5.0和6.2时，分别观察到GIO和的最佳木聚糖酶活性（分别为4.58和3.58 U/ml）。除镁离子外，所有使用的阳离子均提高了木聚糖酶活性。十二烷基硫酸钠对GIO和的木聚糖酶活性支持最高，分别为6.13和6.90 U/ml。在APM上培养的GIO和获得了高产木聚糖酶。木聚糖酶活性受pH值、温度、表面活性剂和阳离子的影响。
