Wang Yanjie, Zhang Ping, Zhang Jingyu, Hong Tie
Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Jilin, Changchun 130021, P.R. China.
Exp Ther Med. 2022 Jun;23(6):401. doi: 10.3892/etm.2022.11328. Epub 2022 Apr 20.
Bisdemethoxycurcumin (BDMC) is an important ingredient derived from turmeric in addition to curcumin. It has been reported that BDMC can be used to treat mast cell-mediated allergic diseases. In the present study, a food allergy (FA) murine model sensitized by intraperitoneal injection followed by oral challenge with ovalbumin (OVA) was established. BDMC was orally administered at 100 and 200 mg/kg for 11 days in the challenge phase to treat OVA-induced FA mice. FA symptoms such as diarrhea score, anaphylactic symptom score and rectal temperature were recorded. Intestinal tissue was also observed by hematoxylin and eosin staining. In addition, other allergic indicators were also analyzed by ELISA and western blot analysis. The present study demonstrated that BDMC could suppress the decreases in rectal temperature, diarrhea and anaphylactic symptoms in FA mice. BDMC could also ameliorate the inflammation of intestinal tissues in FA mice. BDMC not only decreased the production of OVA-specific immunoglobulin (OVA-sIg)E, IgG1, histamine, mouse mast cell protease-1, diamine oxidase, cytokines (IL-4, IL-5 and IL-13) but increased cytokines interferon-γ production. The protein expression results showed that the levels of Gata-3 were decreased but T-bet levels were increased. Furthermore, compared with the OVA group, phosphorylated (p)-p38, p-JNK, p-ERK and p-NF-κBp65 levels were decreased and p-IκBα level was increased. In conclusion, the results showed that BDMC possessed a protective effect on FA. Furthermore, BDMC was able to regulate the T-helper cells (Th)1/Th2 immune balance and inhibit the activation of MAPK and NF-κB pathways in FA mice.
双去甲氧基姜黄素（BDMC）是姜黄中除姜黄素之外的一种重要成分。据报道，BDMC可用于治疗肥大细胞介导的过敏性疾病。在本研究中，通过腹腔注射致敏、随后用卵清蛋白（OVA）口服激发建立了食物过敏（FA）小鼠模型。在激发阶段，以100和200 mg/kg的剂量对OVA诱导的FA小鼠口服给予BDMC，持续11天。记录FA症状，如腹泻评分、过敏症状评分和直肠温度。还通过苏木精-伊红染色观察肠道组织。此外，还通过酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析对其他过敏指标进行了分析。本研究表明，BDMC可抑制FA小鼠直肠温度降低、腹泻和过敏症状。BDMC还可改善FA小鼠肠道组织的炎症。BDMC不仅降低了OVA特异性免疫球蛋白（OVA-sIg）E、IgG1、组胺、小鼠肥大细胞蛋白酶-1、二胺氧化酶、细胞因子（IL-4、IL-5和IL-13）的产生，还增加了细胞因子干扰素-γ的产生。蛋白质表达结果显示，Gata-3水平降低，但T-bet水平升高。此外，与OVA组相比，磷酸化（p）-p38、p-JNK、p-ERK和p-NF-κBp65水平降低，而p-IκBα水平升高。总之，结果表明BDMC对FA具有保护作用。此外，BDMC能够调节辅助性T细胞（Th）1/Th2免疫平衡，并抑制FA小鼠中丝裂原活化蛋白激酶（MAPK）和核因子κB（NF-κB）信号通路的激活。
