Takahashi Hiromi, Hirata Rei, Otsuki Junko, Habara Toshihiro, Hayashi Nobuyoshi
Okayama Couple's Clinic Okayama Japan.
Assisted Reproductive Technology Center Okayama University Okayama Japan.
Reprod Med Biol. 2022 May 23;21(1):e12462. doi: 10.1002/rmb2.12462. eCollection 2022 Jan-Dec.
This study aimed to analyze whether tripronuclear (3PN) zygotes, with two normal-sized PNs and an additional smaller PN (2.1PN), can be used for embryo transfer.
A retrospective embryo cohort study was conducted on 695 patients who underwent intracytoplasmic sperm injection treatment. Blastocyst formation rates were compared between 2.1PN and 2PN zygotes and PGT-A analysis was performed on 15 blastocysts derived from 2.1PN zygotes.
Blastocyst formation rates were comparable between 2.1PN (43.8%) and 2PN zygotes (54.8%; = 0.212). The rates of blastocysts with good morphology derived from 2.1 PN and 2PN zygotes were 18.8% and 25.5%, respectively. No significant differences were detected (= 0.383). All of the analyzed blastocysts were diploid; however, 13 of these were found to be aneuploid, with a further two being mosaic.
Our results suggest that 2.1PN embryos can reach blastocyst stage. These blastocysts were diploid, however, predominantly aneuploid, and therefore could not be used for embryo transfer.
本研究旨在分析具有两个正常大小原核和一个额外较小原核（2.1原核）的三原核（3PN）受精卵是否可用于胚胎移植。
对695例行卵胞浆内单精子注射治疗的患者进行了一项回顾性胚胎队列研究。比较了2.1PN和2PN受精卵的囊胚形成率，并对来自2.1PN受精卵的15个囊胚进行了植入前基因检测分析。
2.1PN受精卵（43.8%）和2PN受精卵（54.8%；P = 0.212）的囊胚形成率相当。来自2.1PN和2PN受精卵的形态良好的囊胚率分别为18.8%和25.5%。未检测到显著差异（P = 0.383）。所有分析的囊胚均为二倍体；然而，其中13个被发现为非整倍体，另外两个为嵌合体。
我们的结果表明，2.1PN胚胎可以发育到囊胚阶段。这些囊胚是二倍体，然而，主要是非整倍体，因此不能用于胚胎移植。
