Zhou Fang-Yu, Quan Ting, Xiang Shi-Jian, Li Hui, Chen Hui-Yuan, Yang Yu-Xuan, Li Sha-Sha
Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, Guangdong, China.
Evid Based Complement Alternat Med. 2022 May 17;2022:9157465. doi: 10.1155/2022/9157465. eCollection 2022.
Radix Fici Hirtae (RFH), known as , is an alternative folk medicine that is widely used to treat various diseases in southern China. The aim of this study was to investigate the effect and metabolic mechanisms of pretreatment with RFH on the serum metabolic profiles of carbon tetrachloride (CCl) induced acute liver injury in mice.
Mice fed with the water extract of RFH at a dose of 1.5 g/kg and 0.75 g/kg for consecutive 7 days, and then serum samples were taken for the metabolomic analysis. Furthermore, the bioinformatics and pathways analysis were measured.
The UHPLC-Orbitrap/MS based-metabolomic analysis identified 20 differential metabolic markers in serum of CCl-induced liver injury mice compared to that of the normal controls, which were mainly related to the metabolism of amino acids and fatty acids. Furthermore, most of these biomarkers contributing to CCl induction were ameliorated by RFH, and the bioinformatics and pathways analysis revealed that therapeutic actions of RFH were mainly involved in the regulation of the oxidative stress responses and energy homeostasis.
These findings provide potential metabolic mechanism for future study and allow for hypothesis generation about the hepatoprotective effects of Radix Fici Hirtae.
五指毛桃，在中国南方作为一种替代民间药物被广泛用于治疗各种疾病。本研究旨在探讨五指毛桃预处理对四氯化碳（CCl）诱导的小鼠急性肝损伤血清代谢谱的影响及代谢机制。
将小鼠分别以1.5 g/kg和0.75 g/kg的剂量连续7天喂食五指毛桃水提取物，然后采集血清样本进行代谢组学分析。此外，还进行了生物信息学和通路分析。
基于超高效液相色谱-轨道阱质谱（UHPLC-Orbitrap/MS）的代谢组学分析确定，与正常对照组相比，CCl诱导的肝损伤小鼠血清中有20种差异代谢标志物，主要与氨基酸和脂肪酸代谢有关。此外，五指毛桃改善了大多数由CCl诱导产生的生物标志物，生物信息学和通路分析表明，五指毛桃的治疗作用主要涉及氧化应激反应和能量稳态的调节。
这些发现为未来的研究提供了潜在的代谢机制，并有助于对五指毛桃的肝保护作用提出假设。
