五指毛桃对昆明小鼠酒精性肝损伤的保肝潜力评估
Assessment of hepatoprotective potential of Radix Fici Hirtae on alcohol-induced liver injury in Kunming mice.
作者信息
Feng Xiaofei, Li Kangxian, Tan Fangming, Zhu Mei, Zhou Jieyi, Lai Yongjun, Zeng Lingfeng, Ye Yingting, Huang Jing, Wu Xiaosong, Li Shasha
机构信息
Department of Pharmacy, the First Affiliated Hospital of Jinan University, Guangzhou 510632, PR China.
出版信息
Biochem Biophys Rep. 2018 Oct 23;16:69-73. doi: 10.1016/j.bbrep.2018.10.003. eCollection 2018 Dec.
OBJECTIVE
The objective of the present study was to investigate the hepatoprotective role of Radix Fici Hirtae on acute alcohol-induced liver injury in mice.
METHODS
The component of Radix Fici Hirtae was extracted using petroleum ether, chloroform, ethyl acetate and n-butanol and divided into three dose groups of high, medium and low according to the clinical man's normal dose of the 50 g crude drug/d (0.83 g/kg body weight). Saline in concentration of 10 mg/mL, 5 mg/mL and 2.5 mg/mL and a dose of mouse lavage (0.2 mL/10 g mouse body weight) were added to the solution. Histopathlogical analysis of liver was performed. Finally, liver protection was validated by examining the effect of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AKP), and lactate dehydrogenase (LDH) on the hepatic function of mice in alcohol-induced liver injury model.
RESULTS
Except for group with saturated n-butyl alcohol, for the rest of the groups, pathological changes of hepatic lipid and inflammatory cells infiltration were alleviated and liver sinus was normal. As compared to model group, the concentrations of AST, ALT, AKP and LDH in chloroform groups and ethyl acetate groups were significantly decreased.
CONCLUSIONS
Extracts of Radix Fici Hirtae are effective for the prevention of alcohol-induced hepatic damage in mice. The results revealed that extracts of Radix Fici Hirtae could be used as hepatoprotective agent.
目的
本研究旨在探讨五指毛桃对小鼠急性酒精性肝损伤的保肝作用。
方法
采用石油醚、氯仿、乙酸乙酯和正丁醇对五指毛桃成分进行提取，并根据临床成人50 g生药/d（0.83 g/kg体重）的正常剂量分为高、中、低三个剂量组。向溶液中加入浓度为10 mg/mL、5 mg/mL和2.5 mg/mL的生理盐水，并以小鼠灌胃剂量（0.2 mL/10 g小鼠体重）给药。对肝脏进行组织病理学分析。最后，通过检测天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（AKP）和乳酸脱氢酶（LDH）对酒精性肝损伤模型小鼠肝功能的影响来验证肝脏保护作用。
结果
除饱和正丁醇组外，其余各组肝脂质病理改变及炎性细胞浸润均减轻，肝窦正常。与模型组相比，氯仿组和乙酸乙酯组的AST、ALT、AKP和LDH浓度显著降低。
结论
五指毛桃提取物对预防小鼠酒精性肝损伤有效。结果表明，五指毛桃提取物可作为保肝剂。
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